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Suppression of ABCE1 Leads to Decreased Cell Proliferation and Increased Apoptosis in 95-D/NCI-H446 Lung Carcinoma Cells
Alternative TitleSuppression of ABCE1 Leads to Decreased Cell Proliferation and Increased Apoptosis in 95-D/NCI-H446 Lung Carcinoma Cells
Zheng MaoGen1; Gao Ying2; Huang Bo1; Tian DaL1; Yang ChunLu1
2009
Source PublicationPROGRESS IN BIOCHEMISTRY AND BIOPHYSICS
ISSN1000-3282
Volume36Issue:11Pages:1475-1482
AbstractATP-binding cassette protein E (ABCE1) has been annotated as an RNase L inhibitor in eukaryotes. Previous study showed that the overexpression of ABCE1 was related with the occurrence and clinical stage of lung adenocarcinoma. As an initial investigation into the novel functions of ABCE1, siRNA-expressing vectors targeting sites of the ABCE1 gene were constructed from RNAi-Ready pSIREN-DNR-DsRed-Express vector. Cultured 95-D and NCI-H446 lung carcinoma cells were transfected with the siRNA-expressing vectors using FuGENE 6 and transfection efficiency was determined by using fluorescence microscopy. The expression level of ABCE1 protein was determined by Western blot and immunofluorescence staining. Cell viability was determined by MTT, cell cycle was analysed by flow cytometry. The apoptotic rate was observed by ELISA. Fluorescence microscopy showed a satisfactory transfection efficiency which was about 42.70%. Cell viability and the growth fraction were markedly suppressed,whereas the apoptosis was significantly increased in SiRNA-95-D and SiRNA-NCI-H446 cells than controls(P < 0.05). It can be concluded that the siRNA targeting A BCE1 gene shows a dramatic inhibitory effect on RNA transcription and protein expression and a promoting effect on the apoptosis in 95-D/NCI-H446 cells, which offers a reliable base for the further in vivo experiment.
KeywordCASSETTE TRANSPORTERS EXPRESSION CANCER GENE ABCE 1 apoptosis lung carcinoma cells proliferation RNA interference
Indexed ByCSCD
Language英语
Funding Project[National Natural Science Foundation of China] ; [Research Special Foundation of Higher School of Education Bureau of Liaoning]
CSCD IDCSCD:3716647
Citation statistics
Cited Times:6[CSCD]   [CSCD Record]
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157923
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.中国科学院大连化学物理研究所
Recommended Citation
GB/T 7714
Zheng MaoGen,Gao Ying,Huang Bo,et al. Suppression of ABCE1 Leads to Decreased Cell Proliferation and Increased Apoptosis in 95-D/NCI-H446 Lung Carcinoma Cells[J]. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,2009,36(11):1475-1482.
APA Zheng MaoGen,Gao Ying,Huang Bo,Tian DaL,&Yang ChunLu.(2009).Suppression of ABCE1 Leads to Decreased Cell Proliferation and Increased Apoptosis in 95-D/NCI-H446 Lung Carcinoma Cells.PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS,36(11),1475-1482.
MLA Zheng MaoGen,et al."Suppression of ABCE1 Leads to Decreased Cell Proliferation and Increased Apoptosis in 95-D/NCI-H446 Lung Carcinoma Cells".PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS 36.11(2009):1475-1482.
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