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Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone
Alternative TitleRegulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone
Xia Lixin; Xiao Ting; Chen Hongduo; Li Ping; Wang Yakun; Wang He
2008
Source PublicationCHINESE MEDICAL JOURNAL
ISSN0366-6999
Volume121Issue:8Pages:730-734
AbstractBackground Haptoglobin (Hp) is one of the acute-phase proteins. Recent studies have demonstrated that Hp exerts immunoregulatory and anti-inflammatory actions and may be one of the inhibitory factors of immune reactions in the skin. In this study we investigated the regulation of Hp expression in a human keratinocyte cell line HaCaT by various cytokines and glucocorticoid.
Other AbstractBackground Haptoglobin (Hp) is one of the acute-phase proteins. Recent studies have demonstrated that Hp exerts immunoregulatory and anti-inflammatory actions and may be one of the inhibitory factors of immune reactions in the skin. In this study we investigated the regulation of Hp expression in a human keratinocyte cell line HaCaT by various cytokines and glucocorticoid. Methods HaCaT cells were cultured with IL-6 (50 ng/ml), TNF-α (20 ng/ml), IFN-γ (20 ng/ml) or IL-4 (20 ng/ml) with or without 1 μmol/L dexamethasone in 6-well plates for 12, 24 and 48 hours. Both the cells and the supernatants were collected to detect the changes of Hp expression by reverse-transcription PCR, ELISA and immunohistochemistry. Results The results showed that Hp expression were elevated at both the mRNA and protein level by the combination of IL-6, TNF-α or IL-4 with dexamethasone, whereas the three cytokines alone did not upregulate the Hp expression. IFN-γ showed no effect on the Hp expression in HaCaT cells.Conclusions These findings suggest that different inflammatory cytokines as well as glucocorticoid may be involved in the regulation of Hp expression in keratinocytes, and this may be one of the negative feedback mechanisms in inflammatory skin diseases.
KeywordACUTE-PHASE RESPONSE TRANS-RETINOIC ACID GENE RAT ALPHA GAMMA IL-13 MICE SKIN haptoglobin cytokine HaCaT cell dexamethasone
Indexed ByCSCD
Language英语
CSCD IDCSCD:3248937
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157933
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Xia Lixin,Xiao Ting,Chen Hongduo,et al. Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone[J]. CHINESE MEDICAL JOURNAL,2008,121(8):730-734.
APA Xia Lixin,Xiao Ting,Chen Hongduo,Li Ping,Wang Yakun,&Wang He.(2008).Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone.CHINESE MEDICAL JOURNAL,121(8),730-734.
MLA Xia Lixin,et al."Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone".CHINESE MEDICAL JOURNAL 121.8(2008):730-734.
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