Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone

IMR OpenIR

	Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone
其他题名	Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone
	Xia Lixin; Xiao Ting; Chen Hongduo; Li Ping; Wang Yakun; Wang He
	2008
发表期刊	CHINESE MEDICAL JOURNAL
ISSN	0366-6999
卷号	121 期号:8 页码:730-734
摘要	Background Haptoglobin (Hp) is one of the acute-phase proteins. Recent studies have demonstrated that Hp exerts immunoregulatory and anti-inflammatory actions and may be one of the inhibitory factors of immune reactions in the skin. In this study we investigated the regulation of Hp expression in a human keratinocyte cell line HaCaT by various cytokines and glucocorticoid.
其他摘要	Background Haptoglobin (Hp) is one of the acute-phase proteins. Recent studies have demonstrated that Hp exerts immunoregulatory and anti-inflammatory actions and may be one of the inhibitory factors of immune reactions in the skin. In this study we investigated the regulation of Hp expression in a human keratinocyte cell line HaCaT by various cytokines and glucocorticoid. Methods HaCaT cells were cultured with IL-6 (50 ng/ml), TNF-α (20 ng/ml), IFN-γ (20 ng/ml) or IL-4 (20 ng/ml) with or without 1 μmol/L dexamethasone in 6-well plates for 12, 24 and 48 hours. Both the cells and the supernatants were collected to detect the changes of Hp expression by reverse-transcription PCR, ELISA and immunohistochemistry. Results The results showed that Hp expression were elevated at both the mRNA and protein level by the combination of IL-6, TNF-α or IL-4 with dexamethasone, whereas the three cytokines alone did not upregulate the Hp expression. IFN-γ showed no effect on the Hp expression in HaCaT cells.Conclusions These findings suggest that different inflammatory cytokines as well as glucocorticoid may be involved in the regulation of Hp expression in keratinocytes, and this may be one of the negative feedback mechanisms in inflammatory skin diseases.
关键词	ACUTE-PHASE RESPONSE TRANS-RETINOIC ACID GENE RAT ALPHA GAMMA IL-13 MICE SKIN haptoglobin cytokine HaCaT cell dexamethasone
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3248937
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157933
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Xia Lixin,Xiao Ting,Chen Hongduo,et al. Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone[J]. CHINESE MEDICAL JOURNAL,2008,121(8):730-734.
APA	Xia Lixin,Xiao Ting,Chen Hongduo,Li Ping,Wang Yakun,&Wang He.(2008).Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone.CHINESE MEDICAL JOURNAL,121(8),730-734.
MLA	Xia Lixin,et al."Regulation of haptoglobin expression in a human keratinocyte cell line HaCaT by inflammatory cytokines and dexamethasone".CHINESE MEDICAL JOURNAL 121.8(2008):730-734.