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MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells
Alternative TitleMicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells
Cao Sunqiong; Ren Changshan
2008
Source PublicationCHINESE JOURNAL OF CANCER RESEARCH
ISSN1000-9604
Volume20Issue:3Pages:159-163
AbstractObjective: To research the silence of NBS1 after transfection microRNA expressing eukaryotic recombinants and the changes of telomerase activation in telomerase-positive cell line Hela. Methods: According to the sequence of NBS1 mRNA, the NBS1 pre-microRNA was designed and synthesized, then cloned into the GFP reporter pcDNA6.2-GW/EmGFP-miR vector and transfected into Hela cells. The integrity of the insert fragment was verified through colony PCR and sequencing analysis. The NBS1 gene expression of NBS1 microRNA recombinants was detected by Real-Time PCR and western blot. Telomerase activity in Hela cells was assayed by TRAP-PCR-EB. Results: Sequences of insert fragment in microRNA expressing recombinants were correct. The NBS1 gene expression was decreased, and the telomerase activation of Hela cell reduced. Conclusion: NBS1 microRNA inhibits NBS1 gene expression, and depresses telomerase activation of Hela cells. This confirms that there is relevance between NBS1 gene and telomerase activity.
Other AbstractObjective To research the silence of NBS1 after transfection microRNA expressing eukaryotic recombinants and the changes of telomerase activation in telomerase-positive cell line Hela.Methods According to the sequence of NBS1 mRNA, the NBS1 pre-microRNA was designed and synthesized, then cloned into the GFP reporter pcDNA6.2-GW/EmGFP-miR vector and transfected into Hela cells. The integrity of the insert fragment was verified through colony PCR and sequencing analysis. The NBS1 gene expression of NBS1 microRNA recombinants was detected by Real-Time PCR and western blot. Telomerase activity in Hela cells was assayed by TRAP-PCR-EB.Results Sequences of insert fragment in microRNA expressing recombinants were correct. The NBS1 gene expression was decreased, and the telomerase activation of Hela cell reduced.Conclusion NBS1 microRNA inhibits NBS1 gene expression, and depresses telomerase activation of Hela cells. This confirms that there is relevance between NBS1 gene and telomerase activity.
KeywordOVEREXPRESSION TRANSFORMATION CONTRIBUTES CANCER TRF1 NBS1 gene RNA interfere MicroRNA Telomerase activation
Indexed ByCSCD
Language英语
CSCD IDCSCD:3360134
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157957
Collection中国科学院金属研究所
Affiliation中国科学院金属研究所
Recommended Citation
GB/T 7714
Cao Sunqiong,Ren Changshan. MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells[J]. CHINESE JOURNAL OF CANCER RESEARCH,2008,20(3):159-163.
APA Cao Sunqiong,&Ren Changshan.(2008).MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells.CHINESE JOURNAL OF CANCER RESEARCH,20(3),159-163.
MLA Cao Sunqiong,et al."MicroRNA-mediated NBS1 gene silence and its effects on telomerase activation in Hela cells".CHINESE JOURNAL OF CANCER RESEARCH 20.3(2008):159-163.
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