Biological Effects of a Three-Dimensionally Printed Ti6Al4V Scaffold Coated with Piezoelectric BaTiO3 Nanoparticles on Bone Formation

doi:10.1021/acsami.0c10957

IMR OpenIR

	Biological Effects of a Three-Dimensionally Printed Ti6Al4V Scaffold Coated with Piezoelectric BaTiO3 Nanoparticles on Bone Formation
	Liu, Wenwen 1; Li, Xiaokang 1; Jiao, Yilai 2; Wu, Cong 3; Guo, Shuo 1; Xiao, Xin 1; Wei, Xinghui 1; Wu, Jie 4; Gao, Peng 5,6; Wang, Ning 1; Lu, Yajie 1; Tang, Zhen 1; Zhao, Quanming 1; Zhang, Jinsong 1; Tang, Yufei 3; Shi, Lei 1; Guo, Zheng 1
通讯作者	Tang, Yufei(guozheng@fmmu.edu.cn) ; Shi, Lei(shilei_med@163.com) ; Guo, Zheng(yftang@xaut.edu.cn)
	2020-11-18
发表期刊	ACS APPLIED MATERIALS & INTERFACES
ISSN	1944-8244
卷号	12 期号:46 页码:51885-51903
摘要	Bone defect repair at load-bearing sites is a challenging clinical problem for orthopedists. Defect reconstruction with implants is the most common treatment; however, it requires the implant to have good mechanical properties and the capacity to promote bone formation. In recent years, the piezoelectric effect, in which electrical activity can be generated due to mechanical deformation, of native bone, which promotes bone formation, has been increasingly valued. Therefore, implants with piezoelectric effects have also attracted great attention from orthopedists. In this study, we developed a bioactive composite scaffold consisting of BaTiO3, a piezoelectric ceramic material, coated on porous Ti6Al4V. This composite scaffold showed not only appropriate mechanical properties, sufficient bone and blood vessel ingrowth space, and a suitable material surface topography but also a reconstructed electromagnetic microenvironment. The osteoconductive and osteoinductive properties of the scaffold were reflected by the proliferation, migration, and osteogenic differentiation of mesenchymal stem cells. The ability of the scaffold to support vascularization was reflected by the proliferation and migration of human umbilical vein endothelial cells and their secretion of VEGF and PDGF-BB. A well-established sheep spinal fusion model was used to evaluate bony fusion in vivo. Sheep underwent implantation with different scaffolds, and X-ray, micro-computed tomography, van Gieson staining, and elemental energy-dispersive spectroscopy were used to analyze bone formation. Isolated cervical angiography and visualization analysis were used to assess angiogenesis at 4 and 8 months after transplantation. The results of cellular and animal studies showed that the piezoelectric effect could significantly reinforce osteogenesis and angiogenesis. Furthermore, we also discuss the molecular mechanism by which the piezoelectric effect promotes osteogenic differentiation and vascularization. In summary, Ti6Al4V scaffold coated with BaTiO3 is a promising composite biomaterial for repairing bone defects, especially at load-bearing sites, that may have great clinical translation potential.
关键词	tissue engineering porous Ti6Al4V scaffold barium titanate piezoelectric effect osteogenesis angiogenesis
资助者	National Key R&D Program of China ; National Natural Science Foundation of China ; Incubation Project of the Army's Medical Technology Youth Cultivation Program of China
DOI	10.1021/acsami.0c10957
收录类别	SCI
语种	英语
资助项目	National Key R&D Program of China[2017YFC1104901] ; National Natural Science Foundation of China[51771227] ; National Natural Science Foundation of China[51871239] ; National Natural Science Foundation of China[81772328] ; Incubation Project of the Army's Medical Technology Youth Cultivation Program of China[17QNP021]
WOS研究方向	Science & Technology - Other Topics ; Materials Science
WOS类目	Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
WOS记录号	WOS:000592923100076
出版者	AMER CHEMICAL SOC
引用统计	被引频次：81[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/158967
专题	中国科学院金属研究所
通讯作者	Tang, Yufei; Shi, Lei; Guo, Zheng
作者单位	1.Fourth Mil Med Univ, Xijing Hosp, Dept Orthopaed, Xian 710032, Shaanxi, Peoples R China 2.Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Peoples R China 3.Xian Univ Technol, Dept Mat Sci & Engn, Xian 710048, Peoples R China 4.Chinese Peoples Liberat Army Gen Hosp, Dept Orthopaed, Med Ctr 8, Beijing 100091, Peoples R China 5.Hunan Normal Univ, Dept Joint Surg & Sports Med, Hunan Prov Peoples Hosp, Changsha 410016, Peoples R China 6.Hunan Normal Univ, Affiliated Hosp 1, Changsha 410016, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenwen,Li, Xiaokang,Jiao, Yilai,et al. Biological Effects of a Three-Dimensionally Printed Ti6Al4V Scaffold Coated with Piezoelectric BaTiO3 Nanoparticles on Bone Formation[J]. ACS APPLIED MATERIALS & INTERFACES,2020,12(46):51885-51903.
APA	Liu, Wenwen.,Li, Xiaokang.,Jiao, Yilai.,Wu, Cong.,Guo, Shuo.,...&Guo, Zheng.(2020).Biological Effects of a Three-Dimensionally Printed Ti6Al4V Scaffold Coated with Piezoelectric BaTiO3 Nanoparticles on Bone Formation.ACS APPLIED MATERIALS & INTERFACES,12(46),51885-51903.
MLA	Liu, Wenwen,et al."Biological Effects of a Three-Dimensionally Printed Ti6Al4V Scaffold Coated with Piezoelectric BaTiO3 Nanoparticles on Bone Formation".ACS APPLIED MATERIALS & INTERFACES 12.46(2020):51885-51903.