Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound

doi:10.1016/j.bioactmat.2021.04.016

IMR OpenIR

	Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound
	Cai, Kunzhan 1; Jiao, Yilai 2; Quan, Quan 1; Hao, Yulin 2; Liu, Jie 3; Wu, Lin 1
通讯作者	Wu, Lin(wulin13@163.com)
	2021-11-01
发表期刊	BIOACTIVE MATERIALS
卷号	6 期号:11 页码:4073-4082
摘要	Developing bioactive materials for bone implants to enhance bone healing and bone growth has for years been the focus of clinical research. Barium titanate (BT) is an electroactive material that can generate electrical signals in response to applied mechanical forces. In this study, a BT piezoelectric ceramic coating was synthesized on the surface of a TC4 titanium alloy, forming a BT/TC4 material, and low-intensity pulsed ultrasound (LIPUS) was then applied as a mechanical stimulus. The combined effects on the biological responses of MC3T3-E1 cells were investigated. Results of scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction showed that an uniform nanospheres -shaped BT coating was formed on TC4 substrate. Piezoelectric behaviors were observed using piezoelectric force microscopy with the piezoelectric coefficient d(33) of 0.42 pC/N. Electrochemical measures indicated that LIPUS-stimulated BT/TC4 materials could produce a microcurrent of approximately 10 mu A/cm(2). In vitro, the greatest osteogenesis (cell adhesion, proliferation, and osteogenic differentiation) was found in MC3T3-E1 cells when BT/TC4 was stimulated using LIPUS. Furthermore, the intracellular calcium ion concentration increased in these cells, possibly because opening of the L-type calcium ion channels was promoted and expression of the Ca(V)1.2 protein was increased. Therefore, the piezoelectric BT/TC4 material with LIPUS loading synergistically promoted osteogenesis, rending it a potential treatment for early stage formation of reliable bone-implant contact.
关键词	Barium titanate Low-intensity pulsed ultrasound Osteogenesis Piezoelectric effect
资助者	National Natural Science Foundation of China ; Natural Science Foundation of Liaoning Province, China ; Scientists Partner Project of China Medical University-Shenyang Branch of Chinese Academy of Sciences
DOI	10.1016/j.bioactmat.2021.04.016
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81870811] ; Natural Science Foundation of Liaoning Province, China[20180530082] ; Scientists Partner Project of China Medical University-Shenyang Branch of Chinese Academy of Sciences[HZHB2018017]
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000685107200030
出版者	KEAI PUBLISHING LTD
引用统计	被引频次：52[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/159236
专题	中国科学院金属研究所
通讯作者	Wu, Lin
作者单位	1.China Med Univ, Sch & Hosp Stomatol, Liaoning Prov Key Lab Oral Dis, Shenyang 110001, Peoples R China 2.Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Peoples R China 3.China Med Univ, Dept Sci Expt Ctr, Shenyang 110122, Peoples R China
推荐引用方式 GB/T 7714	Cai, Kunzhan,Jiao, Yilai,Quan, Quan,et al. Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound[J]. BIOACTIVE MATERIALS,2021,6(11):4073-4082.
APA	Cai, Kunzhan,Jiao, Yilai,Quan, Quan,Hao, Yulin,Liu, Jie,&Wu, Lin.(2021).Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound.BIOACTIVE MATERIALS,6(11),4073-4082.
MLA	Cai, Kunzhan,et al."Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound".BIOACTIVE MATERIALS 6.11(2021):4073-4082.