Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p

IMR OpenIR

	Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p
其他题名	Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p
	Fei Liu 1; Lanting Hu 2; Yi Pei 1; Ke Zheng 1; Wei Wang 1; Shenglong Li 1; Enduo Qiu 1; Guanning Shang 1; Jiaming Zhang 1; Xiaojing Zhang 1
	2020-01-01
发表期刊	BMC Cancer
ISSN	1471-2407
卷号	20 期号:1
摘要	Background Melanoma is the most aggressive skin cancer that derived from pigment cells, accounting for the majority of the skin-cancer-related deaths. Despite great development and evolution have been made in surgery, radiotherapy and adjuvant chemotherapy, the prognosis of melanoma patients exhibited no significant improvement. Long noncoding RNAs (lncRNAs) are frequently dysregulated and involved in the development of cancers. LncRNA AFAP1-AS1 has been explored in various cancers, whereas its role and regulatory mechanism in melanoma are not well understood. Methods The expression of AFAP1-AS1 was detected by qRT-PCR. CCK-8, colony formation, transwell and western blot assays were performed to investigate the biological role of AFAP1-AS1 in melanoma. Male BALB Results AFAP1-AS1 was highly expressed in melanoma cell lines. Suppression of AFAP1-AS1 impaired cell proliferation, migration, invasion and EMT in melanoma. Moreover, AFAP1-AS1 was a ceRNA of RAI14 by competitively binding with miR-653-5p. Besides, miR-653-5p overexpression or RAI14 inhibition could repress tumor growth. Eventually, rescue assays indicated that the function of AFAP1-AS1 in the cellular process of melanoma was dependent on miR-653-5p and RAI14. Conclusions AFAP1-AS1 exerts its oncogenic function in melanoma by targeting miR-653-5p
其他摘要	Background Melanoma is the most aggressive skin cancer that derived from pigment cells, accounting for the majority of the skin-cancer-related deaths. Despite great development and evolution have been made in surgery, radiotherapy and adjuvant chemotherapy, the prognosis of melanoma patients exhibited no significant improvement. Long noncoding RNAs (lncRNAs) are frequently dysregulated and involved in the development of cancers. LncRNA AFAP1-AS1 has been explored in various cancers, whereas its role and regulatory mechanism in melanoma are not well understood. Methods The expression of AFAP1-AS1 was detected by qRT-PCR. CCK-8, colony formation, transwell and western blot assays were performed to investigate the biological role of AFAP1-AS1 in melanoma. Male BALB Results AFAP1-AS1 was highly expressed in melanoma cell lines. Suppression of AFAP1-AS1 impaired cell proliferation, migration, invasion and EMT in melanoma. Moreover, AFAP1-AS1 was a ceRNA of RAI14 by competitively binding with miR-653-5p. Besides, miR-653-5p overexpression or RAI14 inhibition could repress tumor growth. Eventually, rescue assays indicated that the function of AFAP1-AS1 in the cellular process of melanoma was dependent on miR-653-5p and RAI14. Conclusions AFAP1-AS1 exerts its oncogenic function in melanoma by targeting miR-653-5p
语种	英语
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/159343
专题	中国科学院金属研究所
作者单位	1.中国医科大学 2.Institute of Metal Research, Chinese Academy of Sciences
推荐引用方式 GB/T 7714	Fei Liu,Lanting Hu,Yi Pei,et al. Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p[J]. BMC Cancer,2020,20(1).
APA	Fei Liu.,Lanting Hu.,Yi Pei.,Ke Zheng.,Wei Wang.,...&Xiaojing Zhang.(2020).Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p.BMC Cancer,20(1).
MLA	Fei Liu,et al."Long non-coding RNA AFAP1-AS1 accelerates the progression of melanoma by targeting miR-653-5p".BMC Cancer 20.1(2020).