| Reversion of malignant phenotypes of human glioblastoma cells by β -elemene through β -catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules | |
| Alternative Title | Reversion of malignant phenotypes of human glioblastoma cells by β -elemene through β -catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules |
| Tingzhun Zhu; Xiaoming Li; Lihan Luo; Xiaogang Wang; Zhiqing Li; Peng Xie; Xu Gao; Zhenquan Song; Jingyuan Su; Guobiao Liang | |
| 2015-01-01 | |
| Source Publication | Journal of Translational Medicine
 |
| ISSN | 1479-5876 |
| Volume | 13Issue:1 |
| Abstract | Glioblastoma is the most common and lethal type of primary brain tumor. β -Elemene, a natural plant drug extracted from Curcuma wenyujin , has shown strong anti-tumor effects in various tumors with low toxicity. However, the effects of β -elemene on malignant phenotypes of human glioblastoma cells remain to be elucidated. Here we evaluated the effects of β -elemene on cell proliferation, survival, stemness, differentiation and the epithelial-to-mesenchymal transition (EMT) in vitro and in vivo, and investigated the mechanisms underlying these effects. |
| Other Abstract | Background Glioblastoma is the most common and lethal type of primary brain tumor. β -Elemene, a natural plant drug extracted from Curcuma wenyujin , has shown strong anti-tumor effects in various tumors with low toxicity. However, the effects of β -elemene on malignant phenotypes of human glioblastoma cells remain to be elucidated. Here we evaluated the effects of β -elemene on cell proliferation, survival, stemness, differentiation and the epithelial-to-mesenchymal transition (EMT) in vitro and in vivo, and investigated the mechanisms underlying these effects. Methods Human primary and U87 glioblastoma cells were treated with β -elemene, cell viability was measured using a cell counting kit-8 assay, and treated cells were evaluated by flow cytometry. Western blot analysis was carried out to determine the expression levels of stemness markers, differentiation-related molecules and EMT-related effectors. Transwell assays were performed to further determine EMT of glioblastoma cells. To evaluate the effect of β -elemene on glioblastoma in vivo, we subcutaneously injected glioblastoma cells into the flank of nude mice and then intraperitoneally injected NaCl or β -elemene. The tumor xenograft volumes were measured every 3days and the expression of stemness-, differentiation- and EMT-related effectors was determined by Western blot assays in xenografts. Results β -Elemene inhibited proliferation, promoted apoptosis, impaired invasiveness in glioblastoma cells and suppressed the growth of animal xenografts. The expression levels of the stemness markers CD133 and ATP-binding cassette subfamily G member 2 as well as the mesenchymal markers N-cadherin and β -catenin were significantly downregulated, whereas the expression levels of the differentiation-related effectors glial fibrillary acidic protein, Notch1, and sonic hedgehog as well as the epithelial marker E-cadherin were upregulated by β -elemene in vitro and in vivo. Interestingly, the expression of vimentin was increased by β -elemene in vitro; this result was opposite that for the in vivo procedure. Inhibiting β -catenin enhanced the anti-proliferative, EMT-inhibitory and specific marker expression-regulatory effects of β -elemene. Conclusions β -Elemene reversed malignant phenotypes of human glioblastoma cells through β -catenin-involved regulation of stemness-, differentiation- and EMT-related molecules. β -Elemene represents a potentially valuable agent for glioblastoma therapy. |
| Language | 英语 |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/159347 |
| Collection | 中国科学院金属研究所 |
| Affiliation | Institute of Metal Research, Chinese Academy of Sciences |
| Recommended Citation GB/T 7714 | Tingzhun Zhu,Xiaoming Li,Lihan Luo,et al. Reversion of malignant phenotypes of human glioblastoma cells by β -elemene through β -catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules[J]. Journal of Translational Medicine,2015,13(1). |
| APA | Tingzhun Zhu.,Xiaoming Li.,Lihan Luo.,Xiaogang Wang.,Zhiqing Li.,...&Guobiao Liang.(2015).Reversion of malignant phenotypes of human glioblastoma cells by β -elemene through β -catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules.Journal of Translational Medicine,13(1). |
| MLA | Tingzhun Zhu,et al."Reversion of malignant phenotypes of human glioblastoma cells by β -elemene through β -catenin-mediated regulation of stemness-, differentiation- and epithelial-to-mesenchymal transition-related molecules".Journal of Translational Medicine 13.1(2015). |
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