Adipose-derived stem cells cooperate with fractional carbon dioxide laser in antagonizing photoaging: a potential role of Wnt and β-catenin signaling | |
Alternative Title | Adipose-derived stem cells cooperate with fractional carbon dioxide laser in antagonizing photoaging: a potential role of Wnt and β-catenin signaling |
Xiao Xu1; Hong-yi Wang1; Yu Zhang1; Yang Liu2; Yan-qi Li2; Kai Tao1; Chu-Tse Wu2; Ji-de Jin2; Xiao-yan Liu1 | |
2014-01-01 | |
Source Publication | Cell & Bioscience
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ISSN | 2045-3701 |
Volume | 4Issue:1Pages:24-24 |
Abstract | It is well established that adipose-derived stem cells (ADSCs) produce and secrete cytokines 2 ) laser to facilitate photoaging skin healing process has not been explored. Here, we investigated the impacts of ADSCs on photoaging in a photoaging animal model, its associated mechanisms, and its functional cooperation with fractional CO 2 laser in treatment of photoaging skin. |
Other Abstract | Background It is well established that adipose-derived stem cells (ADSCs) produce and secrete cytokines 2 ) laser to facilitate photoaging skin healing process has not been explored. Here, we investigated the impacts of ADSCs on photoaging in a photoaging animal model, its associated mechanisms, and its functional cooperation with fractional CO 2 laser in treatment of photoaging skin. Results We showed that ADSCs improved dermal thickness and activated the proliferation of dermal fibroblast. We further demonstrated that the combined treatment of ADSCs and fractional CO 2 laser, the latter which is often used to resurface skin and treat wrinkles, had more beneficial effects on the photoaging skin compared with each individual treatment. In our prepared HDF photoaging model, flow cytometry showed that, after adipose derived stem cells conditioned medium (ADSC-CM) co-cultured HDF photoaging model, the cell proliferation rate is higher than UVB irradiation induced HDF modeling (p?0.05). Additionally, the expressions of β-catenin and Wnt3a, which were up-regulated after the transplantation of ADSCs alone or in combination with fractional CO 2 laser treatment. And the expression of wnt3a and β-catenin has the positive correlation with photoaging related protein TGF-β2 and COLI. We also verified these protein expressions in tissue level. In addition, after injected SFRP2 into ADSC-CM co-cultured HDF photoaging model, wnt3a inhibitor, compared with un-intervened group, wnt3a, β-catenin protein level significantly decreased. Conclusion Both ADSCs and fractional CO 2 laser improved photoaging skin at least partially via targeting dermal fibroblast activity which was increased in photoaging skin. The combinatorial use of ADSCs and fractional CO 2 laser synergistically improved the healing process of photoaging skin. Thus, we provide a strong rationale for a combined use of ADSCs and fractional CO 2 laser in treatment of photoaging skin in clinic in the future. Moreover, we provided evidence that the Wnt |
Language | 英语 |
Document Type | 期刊论文 |
Identifier | http://ir.imr.ac.cn/handle/321006/159361 |
Collection | 中国科学院金属研究所 |
Affiliation | 1.中国科学院金属研究所 2.Institute of Radiation Medicine |
Recommended Citation GB/T 7714 | Xiao Xu,Hong-yi Wang,Yu Zhang,et al. Adipose-derived stem cells cooperate with fractional carbon dioxide laser in antagonizing photoaging: a potential role of Wnt and β-catenin signaling[J]. Cell & Bioscience,2014,4(1):24-24. |
APA | Xiao Xu.,Hong-yi Wang.,Yu Zhang.,Yang Liu.,Yan-qi Li.,...&Xiao-yan Liu.(2014).Adipose-derived stem cells cooperate with fractional carbon dioxide laser in antagonizing photoaging: a potential role of Wnt and β-catenin signaling.Cell & Bioscience,4(1),24-24. |
MLA | Xiao Xu,et al."Adipose-derived stem cells cooperate with fractional carbon dioxide laser in antagonizing photoaging: a potential role of Wnt and β-catenin signaling".Cell & Bioscience 4.1(2014):24-24. |
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