| Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration | |
| Wang, Chao1,2; Xu, Duoling1,2; Lin, Ling1,2; Li, Shujun3; Hou, Wentao3; He, Yi1,2; Sheng, Liyuan4; Yi, Chen1,2; Zhang, Xiliu1,2; Li, Hongyu1,2; Li, Yiming1,2; Zhao, Wei1,2; Yu, Dongsheng1,2 | |
| Corresponding Author | Zhao, Wei(zhaowei3@mail.sysu.edu.cn) ; Yu, Dongsheng(yudsh@mail.sysu.edu.cn) |
| 2021-12-01 | |
| Source Publication | MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
 |
| ISSN | 0928-4931 |
| Volume | 131Pages:12 |
| Abstract | Porous Ti6Al4V scaffolds are characterized by high porosity, low elastic modulus, and good osteogenesis and vascularization, which are expected to facilitate the repair of large-scale bone defects in future clinical appli-cations. Ti6Al4V scaffolds are divided into regular and irregular structures according to the pore structure, but the pore structure more capable of promoting bone regeneration and angiogenesis has not yet been reported. The purpose of this study was to explore the optimal pore structure and pore size of the Ti6Al4V porous scaffold for the repair of large-area bone defects and the promotion of vascularization in the early stage of osteogenesis. 7 groups of porous Ti6Al4V scaffolds, named NP, R8, R9, R10, P8, P9 and P10, were fabricated by Electron-beam-melting (EBM). Live/dead staining, immunofluorescence staining, SEM, CCK8, ALP, and PCR were used to detect the adhesion, proliferation, and differentiation of BMSCs on different groups of scaffolds. Hematoxylin-eosin (HE) staining and Van Gieson (VG) staining were used to detect bone regeneration and angiogenesis in vivo. The research results showed that as the pore size of the scaffold increased, the surface area and volume of the scaffold gradually decreased, and cell proliferation ability and cell viability gradually increased. The ability of cells to vascularize on scaffolds with irregular pore sizes was stronger than that on scaffolds with regular pore sizes. Micro-CT 3D reconstruction images showed that bone regeneration was obvious and new blood vessels were thick on the P10 scaffold. HE and VG staining showed that the proportion of bone area on the scaffolds with irregular pores was higher than that on scaffolds with regular pores. P10 had better mechanical properties and were more conducive to bone tissue ingrowth and blood vessel formation, thereby facilitating the repair of large -area bone defects. |
| Keyword | Irregular porous structures Pore size Large-area bone defect Vascularization |
| DOI | 10.1016/j.msec.2021.112499 |
| Indexed By | SCI |
| Language | 英语 |
| WOS Research Area | Materials Science |
| WOS Subject | Materials Science, Biomaterials |
| WOS ID | WOS:000712075300002 |
| Publisher | ELSEVIER |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/167168 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Zhao, Wei; Yu, Dongsheng |
| Affiliation | 1.Sun Yat Sen Univ, Guanghua Sch Stomatol, Hosp Stomatol, Guangzhou 510055, Peoples R China 2.Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou 510050, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China 4.Peking Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China |
| Recommended Citation GB/T 7714 | Wang, Chao,Xu, Duoling,Lin, Ling,et al. Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2021,131:12. |
| APA | Wang, Chao.,Xu, Duoling.,Lin, Ling.,Li, Shujun.,Hou, Wentao.,...&Yu, Dongsheng.(2021).Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,131,12. |
| MLA | Wang, Chao,et al."Large-pore-size Ti6Al4V scaffolds with different pore structures for vascularized bone regeneration".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 131(2021):12. |
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