Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid

doi:10.1016/j.bioactmat.2021.07.007

IMR OpenIR

	Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid
	Liu, Wenjing 1,2; Luo, Haiyun 1; Wei, Qinwei 3; Liu, Jia 1; Wu, Junrong 1; Zhang, Yanli 1; Chen, Lili 4; Ren, Wencai 3; Shao, Longquan 1,2
通讯作者	Shao, Longquan(shaolongquan@smu.edu.cn)
	2022-03-01
发表期刊	BIOACTIVE MATERIALS
卷号	9 页码:92-104
摘要	Graphene oxide (GO) exhibits good mechanical and physicochemical characteristics and has extensive application prospects in bone tissue engineering. However, its effect on angiogenesis is unclear, and its potential toxic effects are heavily disputed. Herein, we found that nanographene oxide (NGO) synthesized by one-step water electrolytic oxidation is smaller and shows superior biocompatibility. Moreover, NGO significantly enhanced angiogenesis in calvarial bone defect areas in vivo, providing a good microenvironment for bone regeneration. Endothelial tip cell differentiation is an important step in the initiation of angiogenesis. We verified that NGO activates endothelial tip cells by coupling with lysophosphatidic acid (LPA) in serum via strong hydrogen bonding interactions, which has not been reported. In addition, the mechanism by which NGO promotes angiogenesis was systematically studied. NGO-coupled LPA activates LPAR6 and facilitates the formation of migratory tip cells via Hippo/Yes-associated protein (YAP) independent of reactive oxygen species (ROS) stimulation or additional complex modifications. These results provide an effective strategy for the application of electrochemically derived NGO and more insight into NGO-mediated angiogenesis.
关键词	Nanographene oxide Lysophosphatidic acid Hydrogen bonding Endothelial tip cell Hippo signalling
资助者	National Natural Science Foundation of China ; Natural Science Foundation of Guangdong Province ; Guangdong Basic and Applied Basic Research Foundation
DOI	10.1016/j.bioactmat.2021.07.007
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[52072167] ; National Natural Science Foundation of China[81900989] ; Natural Science Foundation of Guangdong Province[2019A1515011980] ; Guangdong Basic and Applied Basic Research Foundation[2019A1515110088]
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000736276600001
出版者	KEAI PUBLISHING LTD
引用统计	被引频次：16[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/173816
专题	中国科学院金属研究所
通讯作者	Shao, Longquan
作者单位	1.Southern Med Univ, Stomatol Hosp, Guangzhou 510280, Peoples R China 2.Guangdong Prov Key Lab Construct & Detect Tissue, Guangzhou 510515, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang Natl Lab Mat Sci, Shenyang 110016, Peoples R China 4.Huazhong Univ Sci & Technol, Union Hosp, Tongji Med Coll, Stomatol, Wuhan 430022, Peoples R China
推荐引用方式 GB/T 7714	Liu, Wenjing,Luo, Haiyun,Wei, Qinwei,et al. Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid[J]. BIOACTIVE MATERIALS,2022,9:92-104.
APA	Liu, Wenjing.,Luo, Haiyun.,Wei, Qinwei.,Liu, Jia.,Wu, Junrong.,...&Shao, Longquan.(2022).Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.BIOACTIVE MATERIALS,9,92-104.
MLA	Liu, Wenjing,et al."Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid".BIOACTIVE MATERIALS 9(2022):92-104.