| Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway | |
| Xu, Duoling1,2; Wang, Chao1,2; Wu, Jie1,2; Fu, Yuanxiang3,4; Li, Shujun5; Hou, Wentao5; Lin, Ling1,2; Li, Pei1,2; Yu, Dongsheng1,2; Zhao, Wei1,2 | |
| Corresponding Author | Yu, Dongsheng(yudsh@mail.sysu.edu.cn) ; Zhao, Wei(zhaowei3@mail.sysu.edu.cn) |
| 2022-04-26 | |
| Source Publication | ACS OMEGA
 |
| ISSN | 2470-1343 |
| Volume | 7Issue:16Pages:13546-13556 |
| Abstract | Graphene oxide quantum dots (GOQDs) are considered to be a new method for regulating the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). However, there are few reports on such regulation with different concentrations of GOQDs, and the molecular mechanism has not been fully elucidated. The purposes of this study were, first, to explore the effects of GOQDs on the proliferation and differentiation of BMSCs in vitro and in vivo, and, second, to provide a theoretical basis for the repair of bone defects. Live/Dead staining, EdU staining, immuno-fluorescence staining, alkaline phosphatase (ALP), western blotting, and qT-PCR were used for detecting the proliferation and differentiation of BMSCs after coculture with GOQDs of different concentrations. Hematoxylin and eosin (HE) staining and Van Gieson (VG) staining were used to detect new bone regeneration in vivo. The results showed that low-concentration GOQDs (0.1 and 1 mu g/mL) promoted the proliferation and differentiation of BMSCs. Compared with the 1 mu g/mL GOQD group, the 0.1 mu g/mL GOQD group had better ability to promote the proliferation and differentiation of BMSCs. HE and VG staining results showed the greatest proportion of new bone area on sandblasted, large-grit, and acid-etched (SLA)/GOQD scaffolds. Furthermore, the ratio of active beta-catenin and the phosphorylation level of GSK-3 beta (p-GSK-3 beta) increased after BMSCs treatment with 0.1 mu g/mL GOQDs. Low concentrations of GOQDs improved the osteogenic differentiation ability of BMSCs by activating the Wnt/beta-catenin signaling pathway. |
| Funding Organization | National Natural Science Foundation of China ; Science and Technology Planning Project of Guangdong Province ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation (Climbing Program Special Funds) |
| DOI | 10.1021/acsomega.1c06892 |
| Indexed By | SCI |
| Language | 英语 |
| Funding Project | National Natural Science Foundation of China[81974146] ; National Natural Science Foundation of China[81873711] ; Science and Technology Planning Project of Guangdong Province[2016A020215094] ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation (Climbing Program Special Funds)[pdjh 2022b0012] |
| WOS Research Area | Chemistry |
| WOS Subject | Chemistry, Multidisciplinary |
| WOS ID | WOS:000812740100001 |
| Publisher | AMER CHEMICAL SOC |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/174393 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Yu, Dongsheng; Zhao, Wei |
| Affiliation | 1.Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China 2.Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou 510050, Guangdong, Peoples R China 3.Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China 4.Guizhou Inst Technol, Guizhou Prov Key Lab Energy Chem, Guiyang 550003, Peoples R China 5.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China |
| Recommended Citation GB/T 7714 | Xu, Duoling,Wang, Chao,Wu, Jie,et al. Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway[J]. ACS OMEGA,2022,7(16):13546-13556. |
| APA | Xu, Duoling.,Wang, Chao.,Wu, Jie.,Fu, Yuanxiang.,Li, Shujun.,...&Zhao, Wei.(2022).Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway.ACS OMEGA,7(16),13546-13556. |
| MLA | Xu, Duoling,et al."Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway".ACS OMEGA 7.16(2022):13546-13556. |
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