Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway

doi:10.1021/acsomega.1c06892

IMR OpenIR

	Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway
	Xu, Duoling 1,2; Wang, Chao 1,2; Wu, Jie 1,2; Fu, Yuanxiang 3,4; Li, Shujun 5; Hou, Wentao 5; Lin, Ling 1,2; Li, Pei 1,2; Yu, Dongsheng 1,2; Zhao, Wei 1,2
通讯作者	Yu, Dongsheng(yudsh@mail.sysu.edu.cn) ; Zhao, Wei(zhaowei3@mail.sysu.edu.cn)
	2022-04-26
发表期刊	ACS OMEGA
ISSN	2470-1343
卷号	7 期号:16 页码:13546-13556
摘要	Graphene oxide quantum dots (GOQDs) are considered to be a new method for regulating the proliferation and differentiation of bone marrow mesenchymal stem cells (BMSCs). However, there are few reports on such regulation with different concentrations of GOQDs, and the molecular mechanism has not been fully elucidated. The purposes of this study were, first, to explore the effects of GOQDs on the proliferation and differentiation of BMSCs in vitro and in vivo, and, second, to provide a theoretical basis for the repair of bone defects. Live/Dead staining, EdU staining, immuno-fluorescence staining, alkaline phosphatase (ALP), western blotting, and qT-PCR were used for detecting the proliferation and differentiation of BMSCs after coculture with GOQDs of different concentrations. Hematoxylin and eosin (HE) staining and Van Gieson (VG) staining were used to detect new bone regeneration in vivo. The results showed that low-concentration GOQDs (0.1 and 1 mu g/mL) promoted the proliferation and differentiation of BMSCs. Compared with the 1 mu g/mL GOQD group, the 0.1 mu g/mL GOQD group had better ability to promote the proliferation and differentiation of BMSCs. HE and VG staining results showed the greatest proportion of new bone area on sandblasted, large-grit, and acid-etched (SLA)/GOQD scaffolds. Furthermore, the ratio of active beta-catenin and the phosphorylation level of GSK-3 beta (p-GSK-3 beta) increased after BMSCs treatment with 0.1 mu g/mL GOQDs. Low concentrations of GOQDs improved the osteogenic differentiation ability of BMSCs by activating the Wnt/beta-catenin signaling pathway.
资助者	National Natural Science Foundation of China ; Science and Technology Planning Project of Guangdong Province ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation (Climbing Program Special Funds)
DOI	10.1021/acsomega.1c06892
收录类别	SCI
语种	英语
资助项目	National Natural Science Foundation of China[81974146] ; National Natural Science Foundation of China[81873711] ; Science and Technology Planning Project of Guangdong Province[2016A020215094] ; Special Funds for the Cultivation of Guangdong College Students' Scientific and Technological Innovation (Climbing Program Special Funds)[pdjh 2022b0012]
WOS研究方向	Chemistry
WOS类目	Chemistry, Multidisciplinary
WOS记录号	WOS:000812740100001
出版者	AMER CHEMICAL SOC
引用统计	被引频次：11[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/174393
专题	中国科学院金属研究所
通讯作者	Yu, Dongsheng; Zhao, Wei
作者单位	1.Sun Yat Sen Univ, Hosp Stomatol, Guangzhou 510055, Guangdong, Peoples R China 2.Sun Yat Sen Univ, Guangdong Prov Key Lab Stomatol, Guangzhou 510050, Guangdong, Peoples R China 3.Guizhou Inst Technol, Sch Chem Engn, Guiyang 550003, Peoples R China 4.Guizhou Inst Technol, Guizhou Prov Key Lab Energy Chem, Guiyang 550003, Peoples R China 5.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China
推荐引用方式 GB/T 7714	Xu, Duoling,Wang, Chao,Wu, Jie,et al. Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway[J]. ACS OMEGA,2022,7(16):13546-13556.
APA	Xu, Duoling.,Wang, Chao.,Wu, Jie.,Fu, Yuanxiang.,Li, Shujun.,...&Zhao, Wei.(2022).Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway.ACS OMEGA,7(16),13546-13556.
MLA	Xu, Duoling,et al."Effects of Low-Concentration Graphene Oxide Quantum Dots on Improving the Proliferation and Differentiation Ability of Bone Marrow Mesenchymal Stem Cells through the Wnt/beta-Catenin Signaling Pathway".ACS OMEGA 7.16(2022):13546-13556.