<i>In</i><i> vitro</i> long-term antibacterial performance and mechanism of Zn-doped micro-arc oxidation coatings

doi:10.1016/j.colsurfb.2023.113634

IMR OpenIR

	In vitro long-term antibacterial performance and mechanism of Zn-doped micro-arc oxidation coatings
	Liu, Yuzhi 1; Chen, Changtian 2; Liang, Tao 1; Wang, Yaping 1,2; Zhao, Rongfang 2; Li, Guoqiang 2; Bai, Chunguang 3; Wu, Yuxi 4; Yu, Fanglei 5; Sheng, Liyuan 6; Zhang, Rongfa 2; Zhao, Ying 1
通讯作者	Bai, Chunguang(cgbai@imr.ac.cn) ; Zhang, Rongfa(rfzhang-10@163.com) ; Zhao, Ying(ying.zhao@siat.ac.cn)
	2024
发表期刊	COLLOIDS AND SURFACES B-BIOINTERFACES
ISSN	0927-7765
卷号	233 页码:11
摘要	Micro-arc oxidation (MAO) coatings containing 2.86 wt%, 5.83 wt% and 8.81 wt% Zn (Zn-2.86 wt%, Zn-5.83 wt % and Zn-8.81 wt%) were separately fabricated on Ti6Al4V alloys using EDTA-ZnNa2 electrolytes. In vitro antibacterial examination exhibits that the antibacterial rates of Zn-2.86 wt%, Zn-5.83 wt% and Zn-8.81 wt% against Staphylococcus aureus (S. aureus) are 76.0 %, 100.0 % and 99.2 %, respectively. Reactive oxygen species (ROS) level of MAO samples is significantly higher than that of the untreated Ti6Al4V. Zn-containing coatings especially Zn-5.83 wt% induces the strongest oxidative stress on S. aureus due to relatively high released Zn2+ concentration. Moreover, qPCR analysis shows that MAO samples inhibit the icaADBC transcription and result in the down-regulation of PIA production, thereby mitigating biofilm formation. After immersion in simulated body fluid (SBF) for 3, 8 and 14 d, the antibacterial rate of Zn-5.83 wt% is 84.7 %, 63.2 % and 12.5 % respectively, and ROS level of MAO samples is also significantly higher than that of the untreated Ti6Al4V even after 14 d of immersion, suggesting that the antibacterial performance of MAO samples can last a relatively long immersion period and exhibit large application potential in orthopedic clinic.
关键词	Titanium alloys Micro-arc oxidation Zn-doped coatings Antibacterial performance
资助者	Open Project Program of Anhui Province Key Laboratory of Metallurgical Engineering & Resources Recycling (Anhui University of Technology)
DOI	10.1016/j.colsurfb.2023.113634
收录类别	SCI
语种	英语
资助项目	Open Project Program of Anhui Province Key Laboratory of Metallurgical Engineering & Resources Recycling (Anhui University of Technology)[JCYJ20210324120009026] ; Open Project Program of Anhui Province Key Laboratory of Metallurgical Engineering & Resources Recycling (Anhui University of Technology)[JCYJ20210324102013036] ; [SKF23-04]
WOS研究方向	Biophysics ; Chemistry ; Materials Science
WOS类目	Biophysics ; Chemistry, Physical ; Materials Science, Biomaterials
WOS记录号	WOS:001112711300001
出版者	ELSEVIER
引用统计	被引频次：8[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/177250
专题	中国科学院金属研究所
通讯作者	Bai, Chunguang; Zhang, Rongfa; Zhao, Ying
作者单位	1.Chinese Acad Sci, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China 2.Jiangxi Sci & Technol Normal Univ, Sch Mat & Mech & Elect Engn, Nanchang 330013, Peoples R China 3.Chinese Acad Sci, Inst Met Res, Shenyang 110016, Peoples R China 4.Anhui Univ Technol, Key Lab Met Emiss Reduct & Resources Recycling, Minist Educ, Maanshan 243002, Peoples R China 5.Zhejiang Canwell Med Co Ltd, Jinhua 321000, Peoples R China 6.Peking Univ, Shenzhen Inst, Shenzhen 518057, Peoples R China
推荐引用方式 GB/T 7714	Liu, Yuzhi,Chen, Changtian,Liang, Tao,et al. In vitro long-term antibacterial performance and mechanism of Zn-doped micro-arc oxidation coatings[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2024,233:11.
APA	Liu, Yuzhi.,Chen, Changtian.,Liang, Tao.,Wang, Yaping.,Zhao, Rongfang.,...&Zhao, Ying.(2024).In vitro long-term antibacterial performance and mechanism of Zn-doped micro-arc oxidation coatings.COLLOIDS AND SURFACES B-BIOINTERFACES,233,11.
MLA	Liu, Yuzhi,et al."In vitro long-term antibacterial performance and mechanism of Zn-doped micro-arc oxidation coatings".COLLOIDS AND SURFACES B-BIOINTERFACES 233(2024):11.