Electrochemical DNA biosensor based on conducting polyaniline nanotube array

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	Electrochemical DNA biosensor based on conducting polyaniline nanotube array
	H. X. Chang; Y. Yuan; N. L. Shi; Y. F. Guan
	2007
发表期刊	Analytical Chemistry
ISSN	0003-2700
卷号	79 期号:13 页码:5111-5115
摘要	Most of the recent developments in ultrasensitive detection of nucleic acid are based on the gold nanoparticles and carbon nanotubes as a medium of signal amplification. Here, we present an ultrasensitive electrochemical nucleic acid biosensor using the conducting polyaniline (PANI) nanotube array as the signal enhancement element. The PANI nanotube array of a highly organized structure was fabricated under a well-controlled nanoscale dimension on the graphite electrode using a thin nano-porous layer as a template, and 21-mer oligonucleotide probes were immobilized on these PANI nanotubes. In comparison with gold nanoparticle- or carbon nanotube-based DNA biosensors, our PANI nanotube array-based DNA biosensor could achieve similar sensitivity without catalytic enhancement, purification, or end-opening processing. The electrochemical results showed that the conducting PANI nanotube array had a signal enhancement capability, allowing the DNA biosensor to readily detect the target oligonucleotide at a concentration as low as 1.0 fM (similar to 300 zmol of target molecules). In addition, this biosensor demonstrated good capability of differentiating the perfect matched target oligonucleotide from one-nucleotide mismatched oligonucleotides even at a concentration of 37.59 fM. This detection specificity indicates that this biosensor could be applied to single-nucleotide polymorphism analysis and single-mutation detection.
部门归属	chinese acad sci, inst met res, div mat surface engn, shenyang 110016, peoples r china. chinese acad sci, grad sch, beijing 100080, peoples r china. china med univ, dept biochem & mol biol, shenyang 110001, peoples r china.;chang, hx (reprint author), chinese acad sci, inst met res, div mat surface engn, shenyang 110016, peoples r china;hxchang@imr.ac.cn yfguan55@sina.com
关键词	Electrical Detection Nanoparticle Probes Hybridization
URL	查看原文
WOS记录号	WOS:000247611600047
引用统计	被引频次：185[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/33436
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	H. X. Chang,Y. Yuan,N. L. Shi,et al. Electrochemical DNA biosensor based on conducting polyaniline nanotube array[J]. Analytical Chemistry,2007,79(13):5111-5115.
APA	H. X. Chang,Y. Yuan,N. L. Shi,&Y. F. Guan.(2007).Electrochemical DNA biosensor based on conducting polyaniline nanotube array.Analytical Chemistry,79(13),5111-5115.
MLA	H. X. Chang,et al."Electrochemical DNA biosensor based on conducting polyaniline nanotube array".Analytical Chemistry 79.13(2007):5111-5115.