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Effects of the surface characteristics of nanoporous titanium oxide films on Ti-24Nb-4Zr-8Sn alloy on the initial adhesion of osteoblast-like MG-63 cells
Y. Q. Hao; S. J. Li; X. S. Han; Y. L. Hao; H. J. Ai
2013
Source PublicationExperimental and Therapeutic Medicine
ISSN1792-0981
Volume6Issue:1Pages:241-247
AbstractThe aim of the present study was to investigate the effects of the surface characteristics of nanoporous titanium oxide films, formed by anodization on Ti-24Nb-4Zr-8Sn (Ti2448) alloy, on the early adhesion of osteoblast-like MG-63 cells. Nanoporous titanium oxide films with two different pore sizes (30 and 90 nm) were formed by anodization in NH4F solution on Ti2448 alloy. The surface roughness of the nanoporous titanium oxide films was determined using a Surftest Formtracer and field emission scanning electron microscopy (FESEM). Cell viability was evaluated at different time points using the 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. To investigate the regulatory mechanisms involved in the focal adhesion of osteoblasts to Ti2448 alloy, we quantified the expression levels of integrin beta 1 and paxillin mRNAs on the nanoporous titanium oxide films during early osteoblast adhesion using real-time RT-PCR. Samples with a 30-nm nanoporous film exhibited a greater number of overlapping microporous structures with microprojections compared with the 90-nm nanoporous film samples. The MTT assay indicated that cell viability on the 30-nm nanoporous surface following 24 and 48 h of cell culture was higher than those observed on the unanodized control and 90-nm nanoporous surfaces. Integrin beta 1 mRNA expression levels on the 30-nm nanoporous surface following cell culture for 48 h were also significantly higher compared with those on the unanodized control and 90-nm nanoporous surfaces. The results demonstrated that a 30-nm nanoporous titanium oxide filth on Ti2448 alloy may provide the optimum bioactive implant surface for the. initial adhesion of osteoblasts.
description.department[hao, yuquan ; ai, hongjun] china med univ, sch stomatol, dept prosthodont, shenyang 110002, liaoning, peoples r china. [li, shujun ; hao, yulin] chinese acad sci, inst met res, shenyang natl lab mat sci, shenyang 110016, liaoning, peoples r china. [han, xuesong] chinese peoples armed police forces, liaoning prov corps hosp, dept stomatol, shenyang 110034, liaoning, peoples r china. ; ai, hj (reprint author), china med univ, sch stomatol, dept prosthodont, 117 nanjingbei st, shenyang 110002, liaoning, peoples r china. ; cmu_ahj@126.com
KeywordTitanium Alloy Nanoporous Surface Osteoblasts Early Adhesion Anodization Implants Bone Dentistry Roughness Responses Layers Ca
URL查看原文
Language英语
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/71250
Collection中国科学院金属研究所
Recommended Citation
GB/T 7714
Y. Q. Hao,S. J. Li,X. S. Han,et al. Effects of the surface characteristics of nanoporous titanium oxide films on Ti-24Nb-4Zr-8Sn alloy on the initial adhesion of osteoblast-like MG-63 cells[J]. Experimental and Therapeutic Medicine,2013,6(1):241-247.
APA Y. Q. Hao,S. J. Li,X. S. Han,Y. L. Hao,&H. J. Ai.(2013).Effects of the surface characteristics of nanoporous titanium oxide films on Ti-24Nb-4Zr-8Sn alloy on the initial adhesion of osteoblast-like MG-63 cells.Experimental and Therapeutic Medicine,6(1),241-247.
MLA Y. Q. Hao,et al."Effects of the surface characteristics of nanoporous titanium oxide films on Ti-24Nb-4Zr-8Sn alloy on the initial adhesion of osteoblast-like MG-63 cells".Experimental and Therapeutic Medicine 6.1(2013):241-247.
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