Zr61Ti2CU25Al12 metallic glass for potential use in dental implants: Biocompatibility assessment by in vitro cellular responses

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	Zr61Ti2CU25Al12 metallic glass for potential use in dental implants: Biocompatibility assessment by in vitro cellular responses
	J. Li; L. L. Shi; Z. D. Zhu; Q. He; H. J. Ai; J. Xu
	2013
发表期刊	Materials Science & Engineering C-Materials for Biological Applications
ISSN	0928-4931
卷号	33 期号:4 页码:2113-2121
摘要	In comparison with titanium and its alloys, Zr61Ti2Cu25Al12 (ZT1) bulk metallic glass (BMG) manifests a good combination of high strength, high fracture toughness and lower Young's modulus. To examine its biocompatibility required for potential use in dental implants, this BMG was used as a cell growth subtract for three types of cell lines, L929 fibroblasts, human umbilical vein endothelial cells (HUVEC), and osteoblast-like MG63 cells. For a comparison, these cell lines were in parallel cultured and grown also on commercially pure titanium (CP-Ti) and Ti6-Al4-V alloy (Ti64). Cellular responses on the three metals, including adhesion, morphology and viability, were characterized using the SEM visualization and CCK-8 assay. Furthermore, real-time RT-PCR was used to measure the activity of integrin beta, alkaline phosphatase (ALP) and type I collagen (COL I) in adherent MG63 cells. As indicated, in all cases of three cell lines, no significant differences in the initial attachment and viability/proliferation were found between ZT1, CP-Ti, and Ti64 until 5 d of incubation period. It means that the biocompatibility in cellular response for ZT1 BMG is comparable to Ti and its alloys. For gene expression of integrin beta, ALP and COL I, mRNA level from osteoblast cells grown on ZT1 substrates is significantly higher than that on the CP-Ti and Ti64. It suggests that the adhesion and differentiation of osteoblasts grown on ZT1 are even superior to those on the CP-Ti and Ti64 alloy, then promoting bone formation. The good biocompatibility of ZT1 BMG is associated with the formation of zirconium oxide layer on the surface and good corrosion-resistance in physiological environment. (c) 2013 Elsevier B.V. All rights reserved.
部门归属	[li, jing ; ai, hong-jun] china med univ, sch stomatol, shenyang 110002, peoples r china. [shi, ling-ling ; zhu, zhen-dong ; he, qiang ; xu, jian] chinese acad sci, shenyang natl lab mat sci, inst met res, shenyang 110016, peoples r china. ; ai, hj (reprint author), china med univ, sch stomatol, 117 nanjing north sreet, shenyang 110002, peoples r china. ; aih0620@yahoo.com.cn ; jianxu@imr.ac.cn
关键词	Cytotoxicity Biocompatibility Osteoblast Metallic Glass Zirconium Osteoblast-like Cells Human Mg-63 Osteoblasts Collagen Type-i Titanium-alloys Biomedical Applications Corrosion-resistance Fracture-toughness Oxidative Stress Ion Release Bone
URL	查看原文
语种	英语
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/71316
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	J. Li,L. L. Shi,Z. D. Zhu,et al. Zr61Ti2CU25Al12 metallic glass for potential use in dental implants: Biocompatibility assessment by in vitro cellular responses[J]. Materials Science & Engineering C-Materials for Biological Applications,2013,33(4):2113-2121.
APA	J. Li,L. L. Shi,Z. D. Zhu,Q. He,H. J. Ai,&J. Xu.(2013).Zr61Ti2CU25Al12 metallic glass for potential use in dental implants: Biocompatibility assessment by in vitro cellular responses.Materials Science & Engineering C-Materials for Biological Applications,33(4),2113-2121.
MLA	J. Li,et al."Zr61Ti2CU25Al12 metallic glass for potential use in dental implants: Biocompatibility assessment by in vitro cellular responses".Materials Science & Engineering C-Materials for Biological Applications 33.4(2013):2113-2121.