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Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle
L. M. Li; S. Pan; X. H. Zhou; X. Meng; X. X. Han; Y. B. Ren; K. Yang; Y. F. Guan
2013
Source PublicationPlos One
ISSN1932-6203
Volume8Issue:4
AbstractHigh nitrogen nickel-free austenitic stainless steel (HNNF SS) is one of the biomaterials developed recently for circumventing the in-stent restenosis (ISR) in coronary stent applications. To understand the ISR-resistance mechanism, we have conducted a comparative study of cellular and molecular responses of human umbilical vein endothelial cells (HUVECs) to HNNF SS and 316L SS (nickel-containing austenitic 316L stainless steel) which is the stent material used currently. CCK-8 analysis and flow cytometric analysis were used to assess the cellular responses (proliferation, apoptosis, and cell cycle), and quantitative real-time PCR (qRT-PCR) was used to analyze the gene expression profile of HUVECs exposed to HNNF SS and 316L SS, respectively. Flow cytometry analysis revealed that 316L SS could activate the cellular apoptosis more efficiently and initiate an earlier entry into the S-phase of cell cycle than HNNF SS. At the molecular level, qRT-PCR results showed that the genes regulating cell apoptosis and autophagy were overexpressed on 316L SS. Further examination indicated that nickel released from 316L SS triggered the cell apoptosis via Fas-Caspase8-Caspase3 exogenous pathway. These molecular mechanisms of HUVECs present a good model for elucidating the observed cellular responses. The findings in this study furnish valuable information for understanding the mechanism of ISR-resistance on the cellular and molecular basis as well as for developing new biomedical materials for stent applications.
description.department[li, liming ; pan, shuang ; zhou, xiaohang ; meng, xin ; han, xiaoxi ; guan, yifu] china med univ, dept biochem & mol biol, minist educ, key lab med cell biol, shenyang, peoples r china. [li, liming] northeastern univ, coll sci, inst biotechnol, shenyang, peoples r china. [ren, yibin ; yang, ke] chinese acad sci, inst met res, shenyang 110016, peoples r china. ; guan, yf (reprint author), china med univ, dept biochem & mol biol, minist educ, key lab med cell biol, shenyang, peoples r china. ; yfguan@mail.cmu.edu.cn
KeywordCoronary Stents Endothelial-cells Gene-expression Vivo Corrosion Cross-talk Vitro Mechanisms Release Death Cytotoxicity
URL查看原文
Language英语
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/71319
Collection中国科学院金属研究所
Recommended Citation
GB/T 7714
L. M. Li,S. Pan,X. H. Zhou,et al. Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle[J]. Plos One,2013,8(4).
APA L. M. Li.,S. Pan.,X. H. Zhou.,X. Meng.,X. X. Han.,...&Y. F. Guan.(2013).Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle.Plos One,8(4).
MLA L. M. Li,et al."Reduction of In-Stent Restenosis Risk on Nickel-Free Stainless Steel by Regulating Cell Apoptosis and Cell Cycle".Plos One 8.4(2013).
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