Improving Osteointegration and Osteogenesis of Three-Dimensional Porous Ti6AI4V Scaffolds by Polydopamine-Assisted Biomimetic Hydroxyapatite Coating

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	Improving Osteointegration and Osteogenesis of Three-Dimensional Porous Ti6AI4V Scaffolds by Polydopamine-Assisted Biomimetic Hydroxyapatite Coating
	Y.; Yang Li, W.; Li, X. K.; Zhang, X.; Wang, C. R.; Meng, X. F.; Pei, Y. F.; Fan, X. L.; Lan, P. H.; Wang, C. H.; Li, X. J.; Guo, Z.
	2015
发表期刊	Acs Applied Materials & Interfaces
ISSN	1944-8244
卷号	7 期号:10 页码:5715-5724
摘要	Titanium alloys with various porous structures can be fabricated by advanced additive manufacturing techniques, which are attractive for use as scaffolds for bone defect repair. However, modification of the scaffold surfaces, particularly inner surfaces, is critical to improve the osteointegration of these scaffolds. In this study, a biomimetic approach was employed to construct polydopamine-assisted hydroxyapatite coating (HA/pDA) onto porous Ti6A14V scaffolds fabricated by the electron beam melting method. The surface modification was characterized with the field emission scanning electron microscopy, energy dispersive spectroscopy, water contact angle measurement, and confocal laser scanning microscopy. Attachment and proliferation of MC3T3-E1 cells on the scaffold surface were significantly enhanced by the HA/pDA coating compared to the unmodified surfaces. Additionally, MC3T3-E1 cells grown on the HA/pDA-coated Ti6A14V scaffolds displayed significantly higher expression of runt-related transcription factor-2, alkaline phosphatase, osteocalcin, osteopontin, and collagen type-1 compared with bare Ti6A14V scaffolds after culture for 14 days. Moreover, microcomputed tomography analysis and Van-Gieson staining of histological sections showed that HA/pDA coating on surfaces of porous Ti6A14V scaffolds enhanced osteointegration and significantly promoted bone regeneration after implantation in rabbit femoral condylar defects for 4 and 12 weeks. Therefore, this study provides an alternative to biofunctionalized porous T16A14V scaffolds with improved osteointegration and osteogenesis functions for orthopedic applications.
部门归属	[li, yong ; yang, wei ; li, xiaokang ; wang, cairu ; meng, xiangfei ; pei, yifeng ; fan, xiangli ; lan, pingheng ; wang, chunhui ; li, xiaojie ; guo, zheng] fourth mil med univ, xijing hosp, dept orthoped, xian 710032, shaanxi, peoples r china. [zhang, xing] chinese acad sci, shenyang natl lab mat sci, inst met res, shenyang 110016, liaoning, peoples r china. ; guo, z (reprint author), fourth mil med univ, xijing hosp, dept orthoped, xian 710032, shaanxi, peoples r china. ; guozheng@fmmu.edu.cn
关键词	Porous Titanium Surface Modification Polydopamine Hydroxyapatite Osteogenesis Osteointegration Mussel-inspired Polydopamine Mesenchymal Stem-cells Bone Morphogenetic Protein-2 In-vivo Titanium Immobilization Surfaces Osteoconductivity Differentiation Osteoblast
URL	查看原文
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/73851
专题	中国科学院金属研究所
推荐引用方式 GB/T 7714	Y.,Yang Li, W.,Li, X. K.,et al. Improving Osteointegration and Osteogenesis of Three-Dimensional Porous Ti6AI4V Scaffolds by Polydopamine-Assisted Biomimetic Hydroxyapatite Coating[J]. Acs Applied Materials & Interfaces,2015,7(10):5715-5724.
APA	Y..,Yang Li, W..,Li, X. K..,Zhang, X..,Wang, C. R..,...&Guo, Z..(2015).Improving Osteointegration and Osteogenesis of Three-Dimensional Porous Ti6AI4V Scaffolds by Polydopamine-Assisted Biomimetic Hydroxyapatite Coating.Acs Applied Materials & Interfaces,7(10),5715-5724.
MLA	Y.,et al."Improving Osteointegration and Osteogenesis of Three-Dimensional Porous Ti6AI4V Scaffolds by Polydopamine-Assisted Biomimetic Hydroxyapatite Coating".Acs Applied Materials & Interfaces 7.10(2015):5715-5724.