Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers

IMR OpenIR

	Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers
	Wang, Chun; Huang, Nan; Zhuang, Hao; Jiang, Xin; xjiang@imr.ac.cn
	2015
发表期刊	ACS APPLIED MATERIALS & INTERFACES
ISSN	1944-8244
卷号	7 期号:14 页码:7605-7612
摘要	Zinc oxide (ZnO) is considered to be one of the most promising candidates for the third-generation DNA biosensor because of its good chemical stability, wonderful biocompatibility, easy surface modification, and numerous kinds of nanostructures. In this work, we report a new and simple method to modify ZnO surface for the immobilization of oligonucleotides by electrochemical covalent grafting of diazonium salts. The atomic force microscope, X-ray photoelectron spectroscopy, surface contact angle system, and electrochemical workstation were employed to characterize the functionalization process. Fluorescence results show that this kind of DNA biosensor from covalently linking strategy has an enhanced performance compared to that based on an electrostatic adsorption route. The functionalized ZnO biosensor has the capability to distinguish four-base mismatched, one-base mismatched, and complementary DNA sequences. Moreover, a linear relationship has been observed between the fluorescence intensity and the concentration of the complementary DNA in the solution within the range from 10(-6) to 10(-9) M, offering us a possibility in the qualitative determination of the level of target DNA.
部门归属	[wang, chun ; huang, nan ; jiang, xin] chinese acad sci, inst met res, shenyang natl lab mat sci, shenyang 110016, peoples r china ; [zhuang, hao ; jiang, xin] univ siegen, inst mat engn, d-57076 siegen, germany
关键词	Zno Functionalization Coilalent Bond Electrochemistry Fluorescence Dna Biosensor
资助者	National Natural Science Foundation of China [51202257]; Liaoning Province Doctoral Scientific Research Foundation of China [20131121]
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/74824
专题	中国科学院金属研究所
通讯作者	xjiang@imr.ac.cn
推荐引用方式 GB/T 7714	Wang, Chun,Huang, Nan,Zhuang, Hao,et al. Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers[J]. ACS APPLIED MATERIALS & INTERFACES,2015,7(14):7605-7612.
APA	Wang, Chun,Huang, Nan,Zhuang, Hao,Jiang, Xin,&xjiang@imr.ac.cn.(2015).Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers.ACS APPLIED MATERIALS & INTERFACES,7(14),7605-7612.
MLA	Wang, Chun,et al."Enhanced Performance of Nanocrystalline ZnO DNA Biosensor via Introducing Electrochemical Covalent Biolinkers".ACS APPLIED MATERIALS & INTERFACES 7.14(2015):7605-7612.