Osteogenic ability of Cu-bearing stainless steel

IMR OpenIR

	Osteogenic ability of Cu-bearing stainless steel
	Ren, Ling; Wong, Hoi Man; Yan, Chun Hoi; Yeung, Kelvin W. K.; Yang, Ke; kyang@imr.ac.cn
	2015
发表期刊	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
ISSN	1552-4973
卷号	103 期号:7 页码:1433-1444
摘要	A newly developed copper-bearing stainless steel (Cu-SS) by directly immobilizing proper amount of Cu into a medical stainless steel (317L SS) during the metallurgical process could enable continuous release of trace amount of Cu2+ ions, which play the key role to offer the multibiofunctions of the stainless steel, including the osteogenic ability in the present study. The results of in vitro experiments clearly demonstrated that Cu2+ ions from Cu-SS could promote the osteogenic differentiation by stimulating the Alkaline phosphatase enzyme activity and the osteogenic gene expressions (Col1a1, Opn, and Runx2), and enhancing the adhesion and proliferation of osteoblasts cultured on its surface. The in vivo test further proved that more new bone tissue formed around the Cu-SS implant with more stable bone-to-implant contact in comparison with the 317L SS. In addition, Cu-SS showed satisfied bio- compatibility according to the results of in vitro cytotoxicity and in vivo histocompatibility, and its daily released amount of Cu2- ions in physiological saline solution was at trace level of ppb order (1.4 ppb/cm(2)), which is rather safe to human health. Apart from these results, it was also found that Cu-SS could inhibit the happening of inflammation with lower TNF-(x expression in the bone tissue post implantation compared with 317L SS. In addition to good biocompatibility, the overall findings demonstrated that the Cu-SS possessed obvious ability of promoting osteogenesis, indicating a unique application advantage in orthopedics. (C) 2014 Wiley Periodicals, Inc. J Blamed Mater Res Part B: Appl Biomater, 103B: 1433-1444,2015.
部门归属	[ren, ling] chinese acad sci, inst met res, shenyang 110016, peoples r china ; [wong, hoi man ; yan, chun hoi ; yeung, kelvin w. k. ; yang, ke] univ hong kong, dept orthopaed & traumatol, pokfulam, hong kong, peoples r china ; [wong, hoi man ; yan, chun hoi ; yeung, kelvin w. k.] univ hong kong, shenzhen hosp, shenzhen key lab innovat technol orthopaed trauma, shenzhen, peoples r china
关键词	Stainless Steel Metal Ions Bone Osteogenesis Bioactive Material
资助者	National Basic Research Program of China (973 Program) [2012CB619101]; National Natural Science Foundation of China [81301329]; Shenyang National Lab for Materials Science
收录类别	sci
语种	英语
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/75043
专题	中国科学院金属研究所
通讯作者	kyang@imr.ac.cn
推荐引用方式 GB/T 7714	Ren, Ling,Wong, Hoi Man,Yan, Chun Hoi,et al. Osteogenic ability of Cu-bearing stainless steel[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2015,103(7):1433-1444.
APA	Ren, Ling,Wong, Hoi Man,Yan, Chun Hoi,Yeung, Kelvin W. K.,Yang, Ke,&kyang@imr.ac.cn.(2015).Osteogenic ability of Cu-bearing stainless steel.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,103(7),1433-1444.
MLA	Ren, Ling,et al."Osteogenic ability of Cu-bearing stainless steel".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 103.7(2015):1433-1444.