Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo

IMR OpenIR

	Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo
	Wei, Xiaowei; Zhao, Dewei; Wang, Benjie; Wang, Wei; Kang, Kai; Xie, Hui; Liu, Baoyi; Zhang, Xiuzhi; Zhang, Jinsong; Yang, Zhenming; zhaodewei2000@163.com
	2016
发表期刊	EXPERIMENTAL BIOLOGY AND MEDICINE
ISSN	1535-3702
卷号	241 期号:6 页码:592-602
摘要	Porous tantalum metal with low elastic modulus is similar to cancellous bone. Reticulated vitreous carbon (RVC) can provide three-dimensional pore structure and serves as the ideal scaffold of tantalum coating. In this study, the biocompatibility of domestic porous tantalum was first successfully tested with bone marrow stromal stem cells (BMSCs) in vitro and for bone tissue repair in vivo. We evaluated cytotoxicity of RVC scaffold and tantalum coating using BMSCs. The morphology, adhesion, and proliferation of BMSCs were observed via laser scanning confocal microscope and scanning electron microscopy. In addition, porous tantalum rods with or without autologous BMSCs were implanted on hind legs in dogs, respectively. The osteogenic potential was observed by hard tissue slice examination. At three weeks and six weeks following implantation, new osteoblasts and new bone were observed at the tantalum-host bone interface and pores. At 12 weeks postporous tantalum with autologous BMSCs implantation, regenerated trabecular equivalent to mature bone was found in the pore of tantalum rods. Our results suggested that domestic porous tantalum had excellent biocompatibility and could promote new bone formation in vivo. Meanwhile, the osteogenesis of porous tantalum associated with autologous BMSCs was more excellent than only tantalum implantation. Future clinical studies are warranted to verify the clinical efficacy of combined implantation of this domestic porous tantalum associated with autologous BMSCs implantation and compare their efficacy with conventional autologous bone grafting carrying blood vessel in patients needing bone repairing.
部门归属	[wei, xiaowei ; zhao, dewei ; wang, benjie ; wang, wei ; kang, kai ; xie, hui ; liu, baoyi ; zhang, xiuzhi] dalian univ, dept orthopaed lab, zhongshan hosp, dalian 116001, peoples r china ; [zhang, jinsong ; yang, zhenming] chinese acad sci, inst met res, shenyang 110016, peoples r china
关键词	Bone Marrow Stromal Stem Cells Porous Tantalum Biocompatibility Osteogenesis Bone Tissue Engineering
资助者	National Natural Science Foundation of China [81371942]; National Science and Technology Support Program [2012BAI17B02]; Dalian Science and Technology Bureau [2013J21DW004]; Dalian University [2015YBL003]
收录类别	sci
语种	英语
WOS记录号	WOS:000372203100004
引用统计	被引频次：39[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/75177
专题	中国科学院金属研究所
通讯作者	zhaodewei2000@163.com
推荐引用方式 GB/T 7714	Wei, Xiaowei,Zhao, Dewei,Wang, Benjie,et al. Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo[J]. EXPERIMENTAL BIOLOGY AND MEDICINE,2016,241(6):592-602.
APA	Wei, Xiaowei.,Zhao, Dewei.,Wang, Benjie.,Wang, Wei.,Kang, Kai.,...&zhaodewei2000@163.com.(2016).Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo.EXPERIMENTAL BIOLOGY AND MEDICINE,241(6),592-602.
MLA	Wei, Xiaowei,et al."Tantalum coating of porous carbon scaffold supplemented with autologous bone marrow stromal stem cells for bone regeneration in vitro and in vivo".EXPERIMENTAL BIOLOGY AND MEDICINE 241.6(2016):592-602.