Biocompatibility and neurotoxicity of magnesium alloys potentially used for neural repairs

IMR OpenIR

	Biocompatibility and neurotoxicity of magnesium alloys potentially used for neural repairs
	Fei, Jianjun; Wen, Xiaoxiao; Lin, Xiao; Saijilafu; Wang, Weihua; Ren, Olga; Chen, Xinjian; Tan, Lili; Yang, Ke; Yang, Huilin; Yang, Lei; Lin, X (reprint author), Soochow Univ, Room 318 Bldg 1,South Campus,708 Renmin Rd, Suzhou 215006, Jiangsu, Peoples R China.; Yang, K (reprint author), Inst Met Sci & Technol, 72 Wenhua Rd, Shenyang 110016, Liaoning Provin, Peoples R China.; Yang, L (reprint author), Soochow Univ, Room 313 Bldg 1,South Campus,708 Renmin Rd, Suzhou 215006, Jiangsu, Peoples R China.
	2017-09-01
发表期刊	MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
ISSN	0928-4931
卷号	78 页码:1155-1163
摘要	Nerve injury, especially the large-size nerve damage, is a serious problem affecting millions of people. Entubulation of two ends of the injured nerve by using an implantable device, e.g., nerve guidance conduit (NGC), to guide the regeneration of nerve tissue is a promising approach for treating the large-size nerve defect. Magnesium (Mg) and its alloys are biodegradable, conductive, and own good mechanical properties. Mg2+ ion, one of the main degradation products of Mg and its alloys, was reported to promote the proliferation of neural stem cells and their neurite production. Thus, Mg and its alloys are potential materials for fabricating the nerve repair implants, such as NGC or scaffold. However, the compatibility of Mg alloys to cells, especially neurons is not clear. In this work, NZ20 (Mg-2Nd-Zn), ZN20 (Mg-2Zn-Nd) and Mg-10Li magnesium alloys were selected for study, due to the improved mechanical properties of NZ20 and ZN20 alloys and bio-function of Li+ ions from Mg-10Li to nervous system, respectively. The degradation behavior and biocompatibility were studied by in vitro degradation test and cell adhesion assay, respectively. Specifically, the cytocompatibility to dorsal root ganglion (DRG) neurons, RF/6A choroid-retina endothelial cells, and osteoblasts in the cell culture media containing Mg alloy extracts were investigated. The results showed that Mg alloys degraded at different rates in cell culture media and artificial cerebrospinal fluid. The three alloy extracts showed negligible toxic effects on the endothelial cells and osteoblasts at short term (1 day), while NZ20 extract inhibited the proliferation of these two types of cells. The effect of Mg alloy extracts on cell proliferation was also concentration-dependent. For DRG neurons, ZN20 and Mg-10Li alloy extracts showed no neural toxicity compared with control group. The results of the present work show a potential and feasibility of Mg-10Li and ZN20 for nerve repair applications. (C) 2017 Elsevier B.V. All rights reserved.; Nerve injury, especially the large-size nerve damage, is a serious problem affecting millions of people. Entubulation of two ends of the injured nerve by using an implantable device, e.g., nerve guidance conduit (NGC), to guide the regeneration of nerve tissue is a promising approach for treating the large-size nerve defect. Magnesium (Mg) and its alloys are biodegradable, conductive, and own good mechanical properties. Mg2+ ion, one of the main degradation products of Mg and its alloys, was reported to promote the proliferation of neural stem cells and their neurite production. Thus, Mg and its alloys are potential materials for fabricating the nerve repair implants, such as NGC or scaffold. However, the compatibility of Mg alloys to cells, especially neurons is not clear. In this work, NZ20 (Mg-2Nd-Zn), ZN20 (Mg-2Zn-Nd) and Mg-10Li magnesium alloys were selected for study, due to the improved mechanical properties of NZ20 and ZN20 alloys and bio-function of Li+ ions from Mg-10Li to nervous system, respectively. The degradation behavior and biocompatibility were studied by in vitro degradation test and cell adhesion assay, respectively. Specifically, the cytocompatibility to dorsal root ganglion (DRG) neurons, RF/6A choroid-retina endothelial cells, and osteoblasts in the cell culture media containing Mg alloy extracts were investigated. The results showed that Mg alloys degraded at different rates in cell culture media and artificial cerebrospinal fluid. The three alloy extracts showed negligible toxic effects on the endothelial cells and osteoblasts at short term (1 day), while NZ20 extract inhibited the proliferation of these two types of cells. The effect of Mg alloy extracts on cell proliferation was also concentration-dependent. For DRG neurons, ZN20 and Mg-10Li alloy extracts showed no neural toxicity compared with control group. The results of the present work show a potential and feasibility of Mg-10Li and ZN20 for nerve repair applications. (C) 2017 Elsevier B.V. All rights reserved.
部门归属	[fei, jianjun ; wen, xiaoxiao ; lin, xiao ; saijilafu ; wang, weihua ; yang, huilin ; yang, lei] soochow univ, affiliated hosp 1, dept orthopaed, inst orthopaed, suzhou 215006, jiangsu, peoples r china ; [fei, jianjun ; chen, xinjian] soochow univ, sch elect & informat engn, suzhou 215006, jiangsu, peoples r china ; [saijilafu ; yang, huilin ; yang, lei] soochow univ, ircto, suzhou 215006, jiangsu, peoples r china ; [ren, olga] univ waterloo, honours biomed sci, dept biol, waterloo, on, canada ; [tan, lili ; yang, ke] chinese acad sci, inst met res, shenyang 110016, liaoning provin, peoples r china
关键词	Nerve Repair Magnesium Alloys Biodegradation Dorsal Root Ganglion Biocompatibility
学科领域	Materials Science, bioMaterials
资助者	National Basic Research Program of China (973 Program) [2014CB748600]; Priority Academic Program Development of Jiangsu High Education Institutions (PAPD); Jiangsu Innovation and Entrepreneurship Program; National Natural Science Foundation of China [51672184, 81622032, 81501858, 51472279]; Jiangsu Provincial Special Program of Medical Science [BL2012004]; Jiangsu Six Peak of Talents Program [2013-WSW-056]
收录类别	SCI
语种	英语
WOS记录号	WOS:000404944700133
引用统计	被引频次：35[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/78014
专题	中国科学院金属研究所
通讯作者	Lin, X (reprint author), Soochow Univ, Room 318 Bldg 1,South Campus,708 Renmin Rd, Suzhou 215006, Jiangsu, Peoples R China.; Yang, K (reprint author), Inst Met Sci & Technol, 72 Wenhua Rd, Shenyang 110016, Liaoning Provin, Peoples R China.; Yang, L (reprint author), Soochow Univ, Room 313 Bldg 1,South Campus,708 Renmin Rd, Suzhou 215006, Jiangsu, Peoples R China.
推荐引用方式 GB/T 7714	Fei, Jianjun,Wen, Xiaoxiao,Lin, Xiao,et al. Biocompatibility and neurotoxicity of magnesium alloys potentially used for neural repairs[J]. MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,2017,78:1155-1163.
APA	Fei, Jianjun.,Wen, Xiaoxiao.,Lin, Xiao.,Saijilafu.,Wang, Weihua.,...&Yang, L .(2017).Biocompatibility and neurotoxicity of magnesium alloys potentially used for neural repairs.MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS,78,1155-1163.
MLA	Fei, Jianjun,et al."Biocompatibility and neurotoxicity of magnesium alloys potentially used for neural repairs".MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS 78(2017):1155-1163.