| Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure | |
| Nune, K. C.; Misra, R. D. K.; Li, S. J.; Hao, Y. L.; Yang, R.; Misra, RDK (reprint author), Univ Texas El Paso, Dept Met Mat & Biomed Engn, Biomat & Biomed Engn Res Lab, 500 W Univ Ave, El Paso, TX 79968 USA. | |
| 2017-03-01 | |
| Source Publication | JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
 |
| ISSN | 1549-3296 |
| Volume | 105Issue:3Pages:859-870 |
| Abstract | Titanium alloys (Ti-6Al-4V and Ti-6Al-7Nb) are widely used for implants, which are characterized by high elastic modulus (approximate to 110 GPa) with (+) structure and that may induce undesirable stress shielding effect and immune responses associated with the presence of toxic elements. In this regard, we have combined the attributes of a new alloy design and the concept of additive manufacturing to fabricate 3D scaffolds with an interconnected porous structure. The new alloy is a -type Ti-24Nb-4Zr-8Sn (Ti2448) alloy with significantly reduced modulus. In the present study, we explore the biological response of electron beam melted low modulus Ti2448 alloy porous mesh structure through the elucidation of bioactivity and osteoblast functions. The cellular activity was explored in terms of cell-to-cell communication involving proliferation, spreading, synthesis of extracellular and intracellular proteins, differentiation, and mineralization. The formation of fine apatite-like crystals on the surface during immersion test in simulated body fluid confirmed the bioactivity of the scaffold surface, which provided the favorable osteogenic microenvironment for cell-material interaction. The combination of unique surface chemistry and interconnected porous architecture provided the desired pathway for supply of nutrients and oxygen to cells and a favorable osteogenic micro-environment for incorporation (on-growth and in-growth) of osteoblasts. The proliferation and differentiation of pre-osteoblasts and their ability to form a well mineralized bone-like extracellular matrix (ECM) by secreting bone markers (ALP, calcium, etc.) over the struts of the scaffold point toward the determining role of unique surface chemistry and 3D architecture of the Ti2448 alloy mesh structure in modulating osteoblasts functions. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 859-870, 2017.; Titanium alloys (Ti-6Al-4V and Ti-6Al-7Nb) are widely used for implants, which are characterized by high elastic modulus (approximate to 110 GPa) with (+) structure and that may induce undesirable stress shielding effect and immune responses associated with the presence of toxic elements. In this regard, we have combined the attributes of a new alloy design and the concept of additive manufacturing to fabricate 3D scaffolds with an interconnected porous structure. The new alloy is a -type Ti-24Nb-4Zr-8Sn (Ti2448) alloy with significantly reduced modulus. In the present study, we explore the biological response of electron beam melted low modulus Ti2448 alloy porous mesh structure through the elucidation of bioactivity and osteoblast functions. The cellular activity was explored in terms of cell-to-cell communication involving proliferation, spreading, synthesis of extracellular and intracellular proteins, differentiation, and mineralization. The formation of fine apatite-like crystals on the surface during immersion test in simulated body fluid confirmed the bioactivity of the scaffold surface, which provided the favorable osteogenic microenvironment for cell-material interaction. The combination of unique surface chemistry and interconnected porous architecture provided the desired pathway for supply of nutrients and oxygen to cells and a favorable osteogenic micro-environment for incorporation (on-growth and in-growth) of osteoblasts. The proliferation and differentiation of pre-osteoblasts and their ability to form a well mineralized bone-like extracellular matrix (ECM) by secreting bone markers (ALP, calcium, etc.) over the struts of the scaffold point toward the determining role of unique surface chemistry and 3D architecture of the Ti2448 alloy mesh structure in modulating osteoblasts functions. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 859-870, 2017. |
| description.department | [nune, k. c. ; misra, r. d. k.] univ texas el paso, dept met mat & biomed engn, biomat & biomed engn res lab, 500 w univ ave, el paso, tx 79968 usa ; [li, s. j. ; hao, y. l. ; yang, r.] chinese acad sci, inst met res, shenyang natl lab mat sci, shenyang 110016, peoples r china |
| Keyword | Ti-24nb-4zr-8sn Alloy Low Elastic Modulus Mesh Structure Osteoblasts Functions |
| Subject Area | Engineering, Biomedical ; Materials Science, bioMaterials |
| Funding Organization | Chinese MoST [2015AA033702, 2016YFC1102601]; National Natural Science Foundation of China [51271182]; University of Texas at El Paso |
| Indexed By | SCI |
| Language | 英语 |
| WOS ID | WOS:000393957700017 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/78272 |
| Collection | 中国科学院金属研究所 |
| Corresponding Author | Misra, RDK (reprint author), Univ Texas El Paso, Dept Met Mat & Biomed Engn, Biomat & Biomed Engn Res Lab, 500 W Univ Ave, El Paso, TX 79968 USA. |
| Recommended Citation GB/T 7714 | Nune, K. C.,Misra, R. D. K.,Li, S. J.,et al. Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2017,105(3):859-870. |
| APA | Nune, K. C.,Misra, R. D. K.,Li, S. J.,Hao, Y. L.,Yang, R.,&Misra, RDK .(2017).Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,105(3),859-870. |
| MLA | Nune, K. C.,et al."Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 105.3(2017):859-870. |
| Files in This Item: | There are no files associated with this item. | |||||
Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Edit Comment