Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure

IMR OpenIR

	Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure
	Nune, K. C.; Misra, R. D. K.; Li, S. J.; Hao, Y. L.; Yang, R.; Misra, RDK (reprint author), Univ Texas El Paso, Dept Met Mat & Biomed Engn, Biomat & Biomed Engn Res Lab, 500 W Univ Ave, El Paso, TX 79968 USA.
	2017-03-01
发表期刊	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
ISSN	1549-3296
卷号	105 期号:3 页码:859-870
摘要	Titanium alloys (Ti-6Al-4V and Ti-6Al-7Nb) are widely used for implants, which are characterized by high elastic modulus (approximate to 110 GPa) with (+) structure and that may induce undesirable stress shielding effect and immune responses associated with the presence of toxic elements. In this regard, we have combined the attributes of a new alloy design and the concept of additive manufacturing to fabricate 3D scaffolds with an interconnected porous structure. The new alloy is a -type Ti-24Nb-4Zr-8Sn (Ti2448) alloy with significantly reduced modulus. In the present study, we explore the biological response of electron beam melted low modulus Ti2448 alloy porous mesh structure through the elucidation of bioactivity and osteoblast functions. The cellular activity was explored in terms of cell-to-cell communication involving proliferation, spreading, synthesis of extracellular and intracellular proteins, differentiation, and mineralization. The formation of fine apatite-like crystals on the surface during immersion test in simulated body fluid confirmed the bioactivity of the scaffold surface, which provided the favorable osteogenic microenvironment for cell-material interaction. The combination of unique surface chemistry and interconnected porous architecture provided the desired pathway for supply of nutrients and oxygen to cells and a favorable osteogenic micro-environment for incorporation (on-growth and in-growth) of osteoblasts. The proliferation and differentiation of pre-osteoblasts and their ability to form a well mineralized bone-like extracellular matrix (ECM) by secreting bone markers (ALP, calcium, etc.) over the struts of the scaffold point toward the determining role of unique surface chemistry and 3D architecture of the Ti2448 alloy mesh structure in modulating osteoblasts functions. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 859-870, 2017.; Titanium alloys (Ti-6Al-4V and Ti-6Al-7Nb) are widely used for implants, which are characterized by high elastic modulus (approximate to 110 GPa) with (+) structure and that may induce undesirable stress shielding effect and immune responses associated with the presence of toxic elements. In this regard, we have combined the attributes of a new alloy design and the concept of additive manufacturing to fabricate 3D scaffolds with an interconnected porous structure. The new alloy is a -type Ti-24Nb-4Zr-8Sn (Ti2448) alloy with significantly reduced modulus. In the present study, we explore the biological response of electron beam melted low modulus Ti2448 alloy porous mesh structure through the elucidation of bioactivity and osteoblast functions. The cellular activity was explored in terms of cell-to-cell communication involving proliferation, spreading, synthesis of extracellular and intracellular proteins, differentiation, and mineralization. The formation of fine apatite-like crystals on the surface during immersion test in simulated body fluid confirmed the bioactivity of the scaffold surface, which provided the favorable osteogenic microenvironment for cell-material interaction. The combination of unique surface chemistry and interconnected porous architecture provided the desired pathway for supply of nutrients and oxygen to cells and a favorable osteogenic micro-environment for incorporation (on-growth and in-growth) of osteoblasts. The proliferation and differentiation of pre-osteoblasts and their ability to form a well mineralized bone-like extracellular matrix (ECM) by secreting bone markers (ALP, calcium, etc.) over the struts of the scaffold point toward the determining role of unique surface chemistry and 3D architecture of the Ti2448 alloy mesh structure in modulating osteoblasts functions. (c) 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 105A: 859-870, 2017.
部门归属	[nune, k. c. ; misra, r. d. k.] univ texas el paso, dept met mat & biomed engn, biomat & biomed engn res lab, 500 w univ ave, el paso, tx 79968 usa ; [li, s. j. ; hao, y. l. ; yang, r.] chinese acad sci, inst met res, shenyang natl lab mat sci, shenyang 110016, peoples r china
关键词	Ti-24nb-4zr-8sn Alloy Low Elastic Modulus Mesh Structure Osteoblasts Functions
学科领域	Engineering, Biomedical ; Materials Science, bioMaterials
资助者	Chinese MoST [2015AA033702, 2016YFC1102601]; National Natural Science Foundation of China [51271182]; University of Texas at El Paso
收录类别	SCI
语种	英语
WOS记录号	WOS:000393957700017
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/78272
专题	中国科学院金属研究所
通讯作者	Misra, RDK (reprint author), Univ Texas El Paso, Dept Met Mat & Biomed Engn, Biomat & Biomed Engn Res Lab, 500 W Univ Ave, El Paso, TX 79968 USA.
推荐引用方式 GB/T 7714	Nune, K. C.,Misra, R. D. K.,Li, S. J.,et al. Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,2017,105(3):859-870.
APA	Nune, K. C.,Misra, R. D. K.,Li, S. J.,Hao, Y. L.,Yang, R.,&Misra, RDK .(2017).Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A,105(3),859-870.
MLA	Nune, K. C.,et al."Cellular response of osteoblasts to low modulus Ti-24Nb-4Zr-8Sn alloy mesh structure".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A 105.3(2017):859-870.