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Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing
Nune, K. C.; Kumar, A.; Misra, R. D. K.; Li, S. J.; Hao, Y. L.; Yang, R.; Misra, RDK (reprint author), Univ Texas El Paso, Biomat & Biomed Engn Res Lab, Dept Met Mat & Biomed Engn, 500 W Univ Ave, El Paso, TX 79968 USA.
2017-02-01
Source PublicationCOLLOIDS AND SURFACES B-BIOINTERFACES
ISSN0927-7765
Volume150Pages:78-88
AbstractWe elucidate here the osteoblasts functions and cellular activity in 3D printed interconnected porous architecture of functionally gradient Ti-6Al-4V alloy mesh structures in terms of cell proliferation and growth, distribution of cell nuclei, synthesis of proteins (actin, vinculin, and fibronectin), and calcium deposition. Cell culture studies with pre-osteoblasts indicated that the interconnected porous architecture of functionally gradient mesh arrays was conducive to osteoblast functions. However, there were statistically significant differences in the cellular response depending on the pore size in the functionally gradient structure. The interconnected porous architecture contributed to the distribution of cells from the large pore size (G1) to the small pore size (G3), with consequent synthesis of extracellular matrix and calcium precipitation. The gradient mesh structure significantly impacted cell adhesion and influenced the proliferation stage, such that there was high distribution of cells on struts of the gradient mesh structure. Actin and vinculin showed a significant difference in normalized expression level of protein per cell, which was absent in the case of fibronectin. Osteoblasts present on mesh struts formed a confluent sheet, bridging the pores through numerous cytoplasmic extensions. The gradient mesh structure fabricated by electron beam melting was explored to obtain fundamental insights on cellular activity with respect to osteoblast functions. (C) 2016 Elsevier B.V. All rights reserved.; We elucidate here the osteoblasts functions and cellular activity in 3D printed interconnected porous architecture of functionally gradient Ti-6Al-4V alloy mesh structures in terms of cell proliferation and growth, distribution of cell nuclei, synthesis of proteins (actin, vinculin, and fibronectin), and calcium deposition. Cell culture studies with pre-osteoblasts indicated that the interconnected porous architecture of functionally gradient mesh arrays was conducive to osteoblast functions. However, there were statistically significant differences in the cellular response depending on the pore size in the functionally gradient structure. The interconnected porous architecture contributed to the distribution of cells from the large pore size (G1) to the small pore size (G3), with consequent synthesis of extracellular matrix and calcium precipitation. The gradient mesh structure significantly impacted cell adhesion and influenced the proliferation stage, such that there was high distribution of cells on struts of the gradient mesh structure. Actin and vinculin showed a significant difference in normalized expression level of protein per cell, which was absent in the case of fibronectin. Osteoblasts present on mesh struts formed a confluent sheet, bridging the pores through numerous cytoplasmic extensions. The gradient mesh structure fabricated by electron beam melting was explored to obtain fundamental insights on cellular activity with respect to osteoblast functions. (C) 2016 Elsevier B.V. All rights reserved.
description.department[nune, k. c. ; kumar, a. ; misra, r. d. k.] univ texas el paso, biomat & biomed engn res lab, dept met mat & biomed engn, 500 w univ ave, el paso, tx 79968 usa ; [li, s. j. ; hao, y. l. ; yang, r.] chinese acad sci, inst met res, shenyang natl lab mat sci, 72 wenhua rd, shenyang 110016, peoples r china
KeywordGradient Mesh Structure Ti6al4v Osteoblasts
Subject AreaBiophysics ; Chemistry, Physical ; Materials Science, bioMaterials
Funding OrganizationNational Basic Research Program of China [2012CB933902]; Materials Science and Biomedical Engineering programs of the Department of Metallurgical, Materials, and Biomedical Engineering; Chinese MoST [2015AA033702, 2016YFC1102601]
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/78321
Collection中国科学院金属研究所
Corresponding AuthorMisra, RDK (reprint author), Univ Texas El Paso, Biomat & Biomed Engn Res Lab, Dept Met Mat & Biomed Engn, 500 W Univ Ave, El Paso, TX 79968 USA.
Recommended Citation
GB/T 7714
Nune, K. C.,Kumar, A.,Misra, R. D. K.,et al. Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2017,150:78-88.
APA Nune, K. C..,Kumar, A..,Misra, R. D. K..,Li, S. J..,Hao, Y. L..,...&Misra, RDK .(2017).Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing.COLLOIDS AND SURFACES B-BIOINTERFACES,150,78-88.
MLA Nune, K. C.,et al."Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing".COLLOIDS AND SURFACES B-BIOINTERFACES 150(2017):78-88.
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