Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing

IMR OpenIR

	Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing
	Nune, K. C.; Kumar, A.; Misra, R. D. K.; Li, S. J.; Hao, Y. L.; Yang, R.; Misra, RDK (reprint author), Univ Texas El Paso, Biomat & Biomed Engn Res Lab, Dept Met Mat & Biomed Engn, 500 W Univ Ave, El Paso, TX 79968 USA.
	2017-02-01
发表期刊	COLLOIDS AND SURFACES B-BIOINTERFACES
ISSN	0927-7765
卷号	150 页码:78-88
摘要	We elucidate here the osteoblasts functions and cellular activity in 3D printed interconnected porous architecture of functionally gradient Ti-6Al-4V alloy mesh structures in terms of cell proliferation and growth, distribution of cell nuclei, synthesis of proteins (actin, vinculin, and fibronectin), and calcium deposition. Cell culture studies with pre-osteoblasts indicated that the interconnected porous architecture of functionally gradient mesh arrays was conducive to osteoblast functions. However, there were statistically significant differences in the cellular response depending on the pore size in the functionally gradient structure. The interconnected porous architecture contributed to the distribution of cells from the large pore size (G1) to the small pore size (G3), with consequent synthesis of extracellular matrix and calcium precipitation. The gradient mesh structure significantly impacted cell adhesion and influenced the proliferation stage, such that there was high distribution of cells on struts of the gradient mesh structure. Actin and vinculin showed a significant difference in normalized expression level of protein per cell, which was absent in the case of fibronectin. Osteoblasts present on mesh struts formed a confluent sheet, bridging the pores through numerous cytoplasmic extensions. The gradient mesh structure fabricated by electron beam melting was explored to obtain fundamental insights on cellular activity with respect to osteoblast functions. (C) 2016 Elsevier B.V. All rights reserved.; We elucidate here the osteoblasts functions and cellular activity in 3D printed interconnected porous architecture of functionally gradient Ti-6Al-4V alloy mesh structures in terms of cell proliferation and growth, distribution of cell nuclei, synthesis of proteins (actin, vinculin, and fibronectin), and calcium deposition. Cell culture studies with pre-osteoblasts indicated that the interconnected porous architecture of functionally gradient mesh arrays was conducive to osteoblast functions. However, there were statistically significant differences in the cellular response depending on the pore size in the functionally gradient structure. The interconnected porous architecture contributed to the distribution of cells from the large pore size (G1) to the small pore size (G3), with consequent synthesis of extracellular matrix and calcium precipitation. The gradient mesh structure significantly impacted cell adhesion and influenced the proliferation stage, such that there was high distribution of cells on struts of the gradient mesh structure. Actin and vinculin showed a significant difference in normalized expression level of protein per cell, which was absent in the case of fibronectin. Osteoblasts present on mesh struts formed a confluent sheet, bridging the pores through numerous cytoplasmic extensions. The gradient mesh structure fabricated by electron beam melting was explored to obtain fundamental insights on cellular activity with respect to osteoblast functions. (C) 2016 Elsevier B.V. All rights reserved.
部门归属	[nune, k. c. ; kumar, a. ; misra, r. d. k.] univ texas el paso, biomat & biomed engn res lab, dept met mat & biomed engn, 500 w univ ave, el paso, tx 79968 usa ; [li, s. j. ; hao, y. l. ; yang, r.] chinese acad sci, inst met res, shenyang natl lab mat sci, 72 wenhua rd, shenyang 110016, peoples r china
关键词	Gradient Mesh Structure Ti6al4v Osteoblasts
学科领域	Biophysics ; Chemistry, Physical ; Materials Science, bioMaterials
资助者	National Basic Research Program of China [2012CB933902]; Materials Science and Biomedical Engineering programs of the Department of Metallurgical, Materials, and Biomedical Engineering; Chinese MoST [2015AA033702, 2016YFC1102601]
收录类别	SCI
语种	英语
WOS记录号	WOS:000393726900010
引用统计	被引频次：81[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/78321
专题	中国科学院金属研究所
通讯作者	Misra, RDK (reprint author), Univ Texas El Paso, Biomat & Biomed Engn Res Lab, Dept Met Mat & Biomed Engn, 500 W Univ Ave, El Paso, TX 79968 USA.
推荐引用方式 GB/T 7714	Nune, K. C.,Kumar, A.,Misra, R. D. K.,et al. Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2017,150:78-88.
APA	Nune, K. C..,Kumar, A..,Misra, R. D. K..,Li, S. J..,Hao, Y. L..,...&Misra, RDK .(2017).Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing.COLLOIDS AND SURFACES B-BIOINTERFACES,150,78-88.
MLA	Nune, K. C.,et al."Functional response of osteoblasts in functionally gradient titanium alloy mesh arrays processed by 3D additive manufacturing".COLLOIDS AND SURFACES B-BIOINTERFACES 150(2017):78-88.