Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers

IMR OpenIR

	Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers
	Xie, Bei; Yi, Jipeng; Peng, Jian; Zhang, Xing; Lei, Lei; Zhao, Dapeng; Lei, Zhixin; Nie, Hemin; Nie, HM (reprint author), Hunan Univ, Inst Bionanotechnol & Tissue Engn, Coll Life Sci, Changsha 410082, Hunan, Peoples R China.; Zhang, X (reprint author), Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China.
	2017-08-01
发表期刊	JOURNAL MATER SCI TECHNOL
ISSN	1005-0302
卷号	33 期号:8 页码:807-814
摘要	Co-delivery of chemical drugs and therapeutic genes for synergistic therapy provides a promising strategy to treat devastating diseases. However, the real-time coordination patterns between chemical drugs and therapeutic genes remain poorly understood. Herein, the complexes of doxorubicin/graphene oxidepolyethyleneimine/p53 plasmid (Dox/GO-PEI/p53) were fabricated and employed to investigate the synergistic manner between Dox and p53 in the inhibition of HeLa cell growth. GO was conjugated with PEI to form the GO-PEI backbone as the delivery vector. The GO backbone provided surfaces with a high specific area to load Dox via the pi-pi stacking interaction, and was able to release Dox significantly faster at pH 5.0 than at pH 7.0, while the positively charged PEI section of GO-PEI could condense plasmids into GO-PEI/DNA nanoparticles via the electrostatic interaction. The nanoparticles efficiently mediated the transfection of DNA in HeLa cells, with lower cytotoxicity compared to PEI/DNA nanoparticles. Furthermore, the complexes of Dox/GO-PEI/p53 released Dox and expressed p53 gene in a sequential manner, and showed successive inhibition of the in vitro growth of HeLa cells. This type of drug/GO-PEI/DNA complex can be employed as a platform to investigate the coordination pattern between chemical drugs and therapeutic genes for tumor therapy. (C) 2017 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.; Co-delivery of chemical drugs and therapeutic genes for synergistic therapy provides a promising strategy to treat devastating diseases. However, the real-time coordination patterns between chemical drugs and therapeutic genes remain poorly understood. Herein, the complexes of doxorubicin/graphene oxidepolyethyleneimine/p53 plasmid (Dox/GO-PEI/p53) were fabricated and employed to investigate the synergistic manner between Dox and p53 in the inhibition of HeLa cell growth. GO was conjugated with PEI to form the GO-PEI backbone as the delivery vector. The GO backbone provided surfaces with a high specific area to load Dox via the pi-pi stacking interaction, and was able to release Dox significantly faster at pH 5.0 than at pH 7.0, while the positively charged PEI section of GO-PEI could condense plasmids into GO-PEI/DNA nanoparticles via the electrostatic interaction. The nanoparticles efficiently mediated the transfection of DNA in HeLa cells, with lower cytotoxicity compared to PEI/DNA nanoparticles. Furthermore, the complexes of Dox/GO-PEI/p53 released Dox and expressed p53 gene in a sequential manner, and showed successive inhibition of the in vitro growth of HeLa cells. This type of drug/GO-PEI/DNA complex can be employed as a platform to investigate the coordination pattern between chemical drugs and therapeutic genes for tumor therapy. (C) 2017 Published by Elsevier Ltd on behalf of The editorial office of Journal of Materials Science & Technology.
部门归属	[xie, bei ; yi, jipeng ; peng, jian ; zhao, dapeng ; nie, hemin] hunan univ, inst bionanotechnol & tissue engn, coll life sci, changsha 410082, hunan, peoples r china ; [zhang, xing] chinese acad sci, shenyang natl lab mat sci, inst met res, shenyang 110016, liaoning, peoples r china ; [lei, lei] cent s univ, xiangya stomatol hosp, dept orthodont, changsha 410008, hunan, peoples r china ; [lei, zhixin] changsha dmy med technol co ltd, changsha 410005, hunan, peoples r china ; [nie, hemin] hunan univ, shenzhen res inst, nanshan hi new technol & ind pk, shenzhen 518057, peoples r china
关键词	Graphene Oxide Polyethyleneimine Doxorubicin P53 Successive Inhibition
学科领域	Materials Science, Multidisciplinary ; Metallurgy & Metallurgical Engineering
资助者	National Natural Science Foundation of China [31670997]; Natural Science Foundation of Hunan Province [2015JJ1007]; Basic Research Program of Shenzhen City [JCYJ20160530193417959]
收录类别	SCI
语种	英语
WOS记录号	WOS:000410666000008
引用统计	被引频次：13[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/79160
专题	中国科学院金属研究所
通讯作者	Nie, HM (reprint author), Hunan Univ, Inst Bionanotechnol & Tissue Engn, Coll Life Sci, Changsha 410082, Hunan, Peoples R China.; Zhang, X (reprint author), Chinese Acad Sci, Shenyang Natl Lab Mat Sci, Inst Met Res, Shenyang 110016, Liaoning, Peoples R China.
推荐引用方式 GB/T 7714	Xie, Bei,Yi, Jipeng,Peng, Jian,et al. Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers[J]. JOURNAL MATER SCI TECHNOL,2017,33(8):807-814.
APA	Xie, Bei.,Yi, Jipeng.,Peng, Jian.,Zhang, Xing.,Lei, Lei.,...&Zhang, X .(2017).Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers.JOURNAL MATER SCI TECHNOL,33(8),807-814.
MLA	Xie, Bei,et al."Characterization of synergistic anti-tumor effects of doxorubicin and p53 via graphene oxide-polyethyleneimine nanocarriers".JOURNAL MATER SCI TECHNOL 33.8(2017):807-814.