Surface nanotopography-induced favorable modulation of bioactivity and osteoconductive potential of anodized 3D printed Ti-6Al-4V alloy mesh structure

IMR OpenIR

	Surface nanotopography-induced favorable modulation of bioactivity and osteoconductive potential of anodized 3D printed Ti-6Al-4V alloy mesh structure
	Nune, KC; Misra, RDK; Gai, X; Li, SJ; Hao, YL; Misra, RDK (reprint author), Univ Texas El Paso, 500 W Univ Ave Engn Bldg,Met Engn M201, El Paso, TX 79968 USA.
	2018-03-01
发表期刊	JOURNAL OF BIOMATERIALS APPLICATIONS
ISSN	0885-3282
卷号	32 期号:8 页码:1032-1048
摘要	The objective of the study described here is to fundamentally elucidate the biological response of 3D printed Ti-6Al-4V alloy mesh structures that were surface modified to introduce titania nanotubes with an average pore size of approximate to 80nm via an electrochemical anodization process from the perspective of enhancing bioactivity. The bioactivity of the mesh structures were analyzed through immersion test in simulated body fluid, which confirmed the nucleation and growth of fine globular nanoscale apatite on the nanoporous titania-modified (anodized) mesh structure surface, and agglomerated apatite with fine flakes of apatite crystals on as-fabricated mesh structure surface, that were rich in calcium and phosphorous. The cellular activity of bioactive anodized mesh structure was explored in terms of cell-material interactions involving adhesion, proliferation, synthesis of extracellular and intracellular proteins, differentiation, and mineralization. Cells adhered with a sheet-like morphology on as-fabricated mesh structure, whereas, on anodized mesh structure, numerous filopodia-like cellular extensions interacting with nanotube pores were observed. The formation of a bioactive nanoscale apatite, cell-nanotube interactions as imaged via electron microscopy, higher expression of proteins (actin, vinculin, fibronectin, and alkaline phosphatase (ALP)), and calcium content points toward the determining role of anodized mesh structure in modulating osteoblasts functions. The unique combination of nanoporous bioactive titania and interconnected porous architecture of anodized titanium alloy mesh structure provided a multimodal roughness surface ranging from nano to micro to macroscale, which helps in attaining strong primary and secondary fixation of the implant device along with the pathway for supply of nutrients and oxygen to cells and tissue.; The objective of the study described here is to fundamentally elucidate the biological response of 3D printed Ti-6Al-4V alloy mesh structures that were surface modified to introduce titania nanotubes with an average pore size of approximate to 80nm via an electrochemical anodization process from the perspective of enhancing bioactivity. The bioactivity of the mesh structures were analyzed through immersion test in simulated body fluid, which confirmed the nucleation and growth of fine globular nanoscale apatite on the nanoporous titania-modified (anodized) mesh structure surface, and agglomerated apatite with fine flakes of apatite crystals on as-fabricated mesh structure surface, that were rich in calcium and phosphorous. The cellular activity of bioactive anodized mesh structure was explored in terms of cell-material interactions involving adhesion, proliferation, synthesis of extracellular and intracellular proteins, differentiation, and mineralization. Cells adhered with a sheet-like morphology on as-fabricated mesh structure, whereas, on anodized mesh structure, numerous filopodia-like cellular extensions interacting with nanotube pores were observed. The formation of a bioactive nanoscale apatite, cell-nanotube interactions as imaged via electron microscopy, higher expression of proteins (actin, vinculin, fibronectin, and alkaline phosphatase (ALP)), and calcium content points toward the determining role of anodized mesh structure in modulating osteoblasts functions. The unique combination of nanoporous bioactive titania and interconnected porous architecture of anodized titanium alloy mesh structure provided a multimodal roughness surface ranging from nano to micro to macroscale, which helps in attaining strong primary and secondary fixation of the implant device along with the pathway for supply of nutrients and oxygen to cells and tissue.
部门归属	[nune, k. c. ; misra, r. d. k.] univ texas el paso, dept met mat & biomed engn, el paso, tx 79968 usa ; [gai, x. ; li, s. j. ; hao, y. l.] chinese acad sci, inst met res, shenyang natl lab mat sci, shenyang, liaoning, peoples r china
关键词	Titanium-oxide Nanotubes Treated Porous Titanium Micro-arc Oxidation In-vitro Antiinflammatory Properties Biomedical Applications Tio2 Nanotubes Hydrothermal Treatment Osteoblast Functions Functional-response
学科领域	Engineering, Biomedical ; Materials Science, bioMaterials
收录类别	SCI
语种	英语
WOS记录号	WOS:000429860900004
引用统计	被引频次：46[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/79443
专题	中国科学院金属研究所
通讯作者	Misra, RDK (reprint author), Univ Texas El Paso, 500 W Univ Ave Engn Bldg,Met Engn M201, El Paso, TX 79968 USA.
推荐引用方式 GB/T 7714	Nune, KC,Misra, RDK,Gai, X,et al. Surface nanotopography-induced favorable modulation of bioactivity and osteoconductive potential of anodized 3D printed Ti-6Al-4V alloy mesh structure[J]. JOURNAL OF BIOMATERIALS APPLICATIONS,2018,32(8):1032-1048.
APA	Nune, KC,Misra, RDK,Gai, X,Li, SJ,Hao, YL,&Misra, RDK .(2018).Surface nanotopography-induced favorable modulation of bioactivity and osteoconductive potential of anodized 3D printed Ti-6Al-4V alloy mesh structure.JOURNAL OF BIOMATERIALS APPLICATIONS,32(8),1032-1048.
MLA	Nune, KC,et al."Surface nanotopography-induced favorable modulation of bioactivity and osteoconductive potential of anodized 3D printed Ti-6Al-4V alloy mesh structure".JOURNAL OF BIOMATERIALS APPLICATIONS 32.8(2018):1032-1048.