Protective effect of cyclophilin A against Alzheimer's amyloid beta-peptide (25-35)-induced oxidative stress in PC12 cells

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	Protective effect of cyclophilin A against Alzheimer's amyloid beta-peptide (25-35)-induced oxidative stress in PC12 cells
其他题名	Protective effect of cyclophilin A against Alzheimer's amyloid beta-peptide (25-35)-induced oxidative stress in PC12 cells
	Ge Yusong; Teng Weiyu; Zhang Chaodong
	2009
发表期刊	CHINESE MEDICAL JOURNAL
ISSN	0366-6999
卷号	122 期号:6 页码:716-724
摘要	Background beta-amyloid pepticle (A beta) is considered responsible for the pathogenesis of Alzheimer's disease (AD). Possible mechanisms underlying A beta-induced neuronal cytotoxicity include excessive production of reactive oxidative species (ROS) and apoptosis. Cyclophilin A (CypA), exhibits antioxidant properties and protects neurons against oxidative stress induced injury. This study was conducted to demonstrate whether CyPA added to cultured PC12 cells could alleviate Ap-induced oxidative stress and protect them from apoptosis.
其他摘要	Background β-amyloid peptide (Aβ) is considered responsible for the pathogenesis of Alzheimer's disease (AD). Possible mechanisms underlying Aβ-induced neuronal cytotoxicity include excessive production of reactive oxidative species (ROS) and apoptosis. Cyclophilin A (CypA), exhibits antioxidant properties and protects neurons against oxidative stress induced injury. This study was conducted to demonstrate whether CyPA added to cultured PC12 cells could alleviate Aβ-induced oxidative stress and protect them from apoptosis.Methods PC12 cells were pre-incubated for 30 minutes with recombinant human cyclophilin A (rhCyPA) in 0.1 nmol/L, 1.0 nmol/L, 10 nmol/L and 100 nmol/L and then incubated with 10 umol/L Aβ_(25-35). In every group, cell viability, apoptotic morphology, apoptotic rate, intracellular ROS accumulation, the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) of PC12 cells and mitochondrial transmembrane potential were detected. Subsequently, the expression of the active form of caspase-3 was determined by Western blotting.Results It was shown that cultures treated with 1.0 nmol/L, 10 nmol/L or 100 nmol/L rhCyPA + Aβ_(25-35) had significantly higher cell viability and a lower rate of apoptosis compared with the cultures exposed only to Aβ_(25-35). In addition, rhCyPA attenuated Aβ_(25-35)-induced overproduction of intracellular ROS and Aβ_(25-35)-induced a decrease in activity of the key antioxidant enzymes SOD and GSH-Px. Furthermore, rhCyPA also attenuated Aβ_(25-35)-induced mitochondrial dysfunction and the activation of caspase-3.Conclusion CyPA may act as an ROS scavenger, and prevent Aβ_(25-35)-induced neurotoxicity through attenuating oxidative stress induced by Aβ_(25-35).
关键词	TRANS-ISOMERASE ACTIVITY MITOCHONDRIAL DYSFUNCTION A EXPRESSION DIFFERENTIATION ANTIOXIDANT ACTIVATION PROTEIN LOCALIZATION APOPTOSIS PATHWAY Alzheimer's disease cyclophilin A beta-amyloid oxidative stress apoptosis
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:3591628
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/86398
专题	中国科学院金属研究所
作者单位	中国科学院金属研究所
推荐引用方式 GB/T 7714	Ge Yusong,Teng Weiyu,Zhang Chaodong. Protective effect of cyclophilin A against Alzheimer's amyloid beta-peptide (25-35)-induced oxidative stress in PC12 cells[J]. CHINESE MEDICAL JOURNAL,2009,122(6):716-724.
APA	Ge Yusong,Teng Weiyu,&Zhang Chaodong.(2009).Protective effect of cyclophilin A against Alzheimer's amyloid beta-peptide (25-35)-induced oxidative stress in PC12 cells.CHINESE MEDICAL JOURNAL,122(6),716-724.
MLA	Ge Yusong,et al."Protective effect of cyclophilin A against Alzheimer's amyloid beta-peptide (25-35)-induced oxidative stress in PC12 cells".CHINESE MEDICAL JOURNAL 122.6(2009):716-724.