Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo

IMR OpenIR

	Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo
	Zhang, Ying 1,2; Wang, Wei 1; Xie, Yubing 1; Yu, Weiting 1; Lv, Guojun 1,2; Guo, Xin 1; Xiong, Ying 1; Ma, Xiaojun 1
通讯作者	Ma, Xiaojun(maxj@dicp.ae.en)
	2008
发表期刊	JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS
ISSN	1552-4973
卷号	84B 期号:1 页码:79-88
摘要	Microencapsulation of recombinant cells secreting endostatin offers a promising approach to tumor gene therapy in which therapeutic protein is delivered in a sustainable and long-term fashion by encapsulated recombinant cells. However, the studies of cell growth and protein production in vivo are very limited. In this study, the effects of microencapsulation parameters on in vivo cell growth, endostatin production, and microcapsule stability after implantation in the peritoneal cavity of mice were for the first time investigated. Microcapsules with liquid core reached higher cell density and endostatin production at day 18 than microcapsules with solid core. There was no significant difference in stability whether the core of the microcapsulle was solid or liquid. Decrease in microcapsule size increased the stability of microcapsule. The microcapsules kept intact in the peritoneal cavity of mice after 36 days of implantation when the microcapsules size was 240 mu m in diameter, which gave rise to high endostatin production as well. The optimized microencapsulation conditions for in vivo implantation are liquid core and 240 mu m in diameter. This study provides useful information for antiangiogenic gene therapy to tumors using microencapsulated recombinant cells. (c) 2007 Wiley Periodicals, Inc.
关键词	microencapsulation recombinant cell cell growth endostatin production stability in vivo
收录类别	SCI
语种	英语
WOS研究方向	Engineering ; Materials Science
WOS类目	Engineering, Biomedical ; Materials Science, Biomaterials
WOS记录号	WOS:000251802900010
出版者	WILEY-LISS
引用统计	被引频次：12[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/93234
专题	中国科学院金属研究所
通讯作者	Ma, Xiaojun
作者单位	1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Biomed Mat Engn, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch Chinese Acad Sci, Beijing 100039, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Ying,Wang, Wei,Xie, Yubing,et al. Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo[J]. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,2008,84B(1):79-88.
APA	Zhang, Ying.,Wang, Wei.,Xie, Yubing.,Yu, Weiting.,Lv, Guojun.,...&Ma, Xiaojun.(2008).Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo.JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS,84B(1),79-88.
MLA	Zhang, Ying,et al."Optimization of microencapsulated recombinant CHO cell growth, endostatin production, and stability of microcapsule in vivo".JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS 84B.1(2008):79-88.