| Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells | |
| Alternative Title | Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells |
| Zhang Yahong1; Wu Yingliang1; Tashiro Shinichi3; Onodera Satoshi3; Ikejima Takashi1 | |
| 2011 | |
| Source Publication | ACTA PHARMACOLOGICA SINICA
 |
| ISSN | 1671-4083 |
| Volume | 32Issue:10Pages:1266-1275 |
| Abstract | Aim: To investigate the role of reactive oxygen species (ROS) in oridonin-induced apoptosis and autophagy in HeLa cells. |
| Other Abstract | Aim: To investigate the role of reactive oxygen species (ROS) in oridonin-induced apoptosis and autophagy in HeLa cells. Methods: The cell viability was measured using MTT assay. Morphological changes of apoptosis and autophagy were examined using Hoechst 33258 staining and monodansylcadaverine (MDC) staining, respectively. The mitochondrial membrane potential (ΔΨm) was measured using fluorescent dye rhodamine 123. DCF-induced fluorescence was used to measure the intracellular ROS level. Protein expression was examined using Western blot. Results: Treatment of HeLa cells with oridonin (20–160μmol Conclusion: ROS plays a critical role in oridonin-induced apoptosis and autophagy. |
| Keyword | MITOCHONDRIAL PERMEABILITY TRANSITION OXIDATIVE STRESS MEDIATED AUTOPHAGY CANCER DEATH ACTIVATION MECHANISM EXPRESSION GENERATION INDUCTION oridonin reactive oxygen species apoptosis autophagy Bax Bcl-2 caspase 3 caspase 8 HeLa cell |
| Indexed By | CSCD |
| Language | 英语 |
| Funding Project | [China Postdoctoral Science Foundation] ; [He-nan University] |
| CSCD ID | CSCD:4335577 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/143426 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院金属研究所 2.河南大学 3.Showa Pharmaceut University, Dept Clin & Biomed Sci, Tokyo 1948543, Japan |
| Recommended Citation GB/T 7714 | Zhang Yahong,Wu Yingliang,Tashiro Shinichi,et al. Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells[J]. ACTA PHARMACOLOGICA SINICA,2011,32(10):1266-1275. |
| APA | Zhang Yahong,Wu Yingliang,Tashiro Shinichi,Onodera Satoshi,&Ikejima Takashi.(2011).Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells.ACTA PHARMACOLOGICA SINICA,32(10),1266-1275. |
| MLA | Zhang Yahong,et al."Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells".ACTA PHARMACOLOGICA SINICA 32.10(2011):1266-1275. |
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