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Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Alternative TitleReactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Zhang Yahong1; Wu Yingliang1; Tashiro Shinichi3; Onodera Satoshi3; Ikejima Takashi1
2011
Source PublicationACTA PHARMACOLOGICA SINICA
ISSN1671-4083
Volume32Issue:10Pages:1266-1275
AbstractAim: To investigate the role of reactive oxygen species (ROS) in oridonin-induced apoptosis and autophagy in HeLa cells.
Other AbstractAim: To investigate the role of reactive oxygen species (ROS) in oridonin-induced apoptosis and autophagy in HeLa cells. Methods: The cell viability was measured using MTT assay. Morphological changes of apoptosis and autophagy were examined using Hoechst 33258 staining and monodansylcadaverine (MDC) staining, respectively. The mitochondrial membrane potential (ΔΨm) was measured using fluorescent dye rhodamine 123. DCF-induced fluorescence was used to measure the intracellular ROS level. Protein expression was examined using Western blot. Results: Treatment of HeLa cells with oridonin (20–160μmol Conclusion: ROS plays a critical role in oridonin-induced apoptosis and autophagy.
KeywordMITOCHONDRIAL PERMEABILITY TRANSITION OXIDATIVE STRESS MEDIATED AUTOPHAGY CANCER DEATH ACTIVATION MECHANISM EXPRESSION GENERATION INDUCTION oridonin reactive oxygen species apoptosis autophagy Bax Bcl-2 caspase 3 caspase 8 HeLa cell
Indexed ByCSCD
Language英语
Funding Project[China Postdoctoral Science Foundation] ; [He-nan University]
CSCD IDCSCD:4335577
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Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/143426
Collection中国科学院金属研究所
Affiliation1.中国科学院金属研究所
2.河南大学
3.Showa Pharmaceut University, Dept Clin & Biomed Sci, Tokyo 1948543, Japan
Recommended Citation
GB/T 7714
Zhang Yahong,Wu Yingliang,Tashiro Shinichi,et al. Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells[J]. ACTA PHARMACOLOGICA SINICA,2011,32(10):1266-1275.
APA Zhang Yahong,Wu Yingliang,Tashiro Shinichi,Onodera Satoshi,&Ikejima Takashi.(2011).Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells.ACTA PHARMACOLOGICA SINICA,32(10),1266-1275.
MLA Zhang Yahong,et al."Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells".ACTA PHARMACOLOGICA SINICA 32.10(2011):1266-1275.
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