Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells

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	Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
其他题名	Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
	Zhang Yahong 1; Wu Yingliang 1; Tashiro Shinichi 3; Onodera Satoshi 3; Ikejima Takashi 1
	2011
发表期刊	ACTA PHARMACOLOGICA SINICA
ISSN	1671-4083
卷号	32 期号:10 页码:1266-1275
摘要	Aim: To investigate the role of reactive oxygen species (ROS) in oridonin-induced apoptosis and autophagy in HeLa cells.
其他摘要	Aim: To investigate the role of reactive oxygen species (ROS) in oridonin-induced apoptosis and autophagy in HeLa cells. Methods: The cell viability was measured using MTT assay. Morphological changes of apoptosis and autophagy were examined using Hoechst 33258 staining and monodansylcadaverine (MDC) staining, respectively. The mitochondrial membrane potential (ΔΨm) was measured using fluorescent dye rhodamine 123. DCF-induced fluorescence was used to measure the intracellular ROS level. Protein expression was examined using Western blot. Results: Treatment of HeLa cells with oridonin (20–160μmol Conclusion: ROS plays a critical role in oridonin-induced apoptosis and autophagy.
关键词	MITOCHONDRIAL PERMEABILITY TRANSITION OXIDATIVE STRESS MEDIATED AUTOPHAGY CANCER DEATH ACTIVATION MECHANISM EXPRESSION GENERATION INDUCTION oridonin reactive oxygen species apoptosis autophagy Bax Bcl-2 caspase 3 caspase 8 HeLa cell
收录类别	CSCD
语种	英语
资助项目	[China Postdoctoral Science Foundation] ; [He-nan University]
CSCD记录号	CSCD:4335577
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/143426
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.河南大学 3.Showa Pharmaceut University, Dept Clin & Biomed Sci, Tokyo 1948543, Japan
推荐引用方式 GB/T 7714	Zhang Yahong,Wu Yingliang,Tashiro Shinichi,et al. Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells[J]. ACTA PHARMACOLOGICA SINICA,2011,32(10):1266-1275.
APA	Zhang Yahong,Wu Yingliang,Tashiro Shinichi,Onodera Satoshi,&Ikejima Takashi.(2011).Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells.ACTA PHARMACOLOGICA SINICA,32(10),1266-1275.
MLA	Zhang Yahong,et al."Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells".ACTA PHARMACOLOGICA SINICA 32.10(2011):1266-1275.