Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels

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	Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels
其他题名	Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BK Ca channels and reducing intracellular Ca 2+ levels
	Gai Xiangyun 1; Wei Yuhai 3; Zhang Wei 2; Wuren Tana 2; Wang Yaping 2; Li Zhanqiang 2; Liu Shou 2; Ma Lan 2; Lu Dianxiang 2; Zhou Yi 1; Ge Rili 1
	2015
发表期刊	ACTA PHARMACOLOGICA SINICA
ISSN	1671-4083
卷号	36 期号:5 页码:587-596
摘要	Aim: Sustained pulmonary vasoconstriction as experienced at high altitude can lead to pulmonary hypertension (PH). The main purpose of this study is to investigate the vasorelaxant effect of echinacoside (ECH), a phenylethanoid glycoside from the Tibetan herb Lagotis brevituba Maxim and Cistanche tubulosa, on the pulmonary artery and its potential mechanism.
其他摘要	Aim: Sustained pulmonary vasoconstriction as experienced at high altitude can lead to pulmonary hypertension (PH). The main purpose of this study is to investigate the vasorelaxant effect of echinacoside (ECH), a phenylethanoid glycoside from the Tibetan herb Lagotis brevituba Maxim and Cistanche tubulosa , on the pulmonary artery and its potential mechanism. Methods: Pulmonary arterial rings obtained from male Wistar rats were suspended in organ chambers filled with Krebs-Henseleit solution, and isometric tension was measured using a force transducer. Intracellular Ca 2+ levels were measured in cultured rat pulmonary arterial smooth muscle cells (PASMCs) using Fluo 4-AM. Results: ECH (30–300 μmol 50 in endothelium-denuded rings. This effect was significantly blocked by L-NAME, TEA, and BaCl 2 . However, IMT, 4-AP, and Gli did not inhibit ECH-induced relaxation. Under extracellular Ca 2+ -free conditions, the maximum contraction was reduced to 24.54%±2.97% and 10.60%±2.07% in rings treated with 100 and 300 μmol Conclusion: ECH suppresses NE-induced contraction of rat pulmonary artery via reducing intracellular Ca 2+ levels, and induces its relaxation through the NO-cGMP pathway and opening of K + channels (BK Ca and K IR ).
关键词	ACTIVATED POTASSIUM CHANNELS K+ CHANNELS CALCIUM-CHANNEL Tibetan herb echinacoside pulmonary hypertension high altitude vasorelaxation NO BKCa K-IR artery ring pulmonary arterial smooth muscle cells
收录类别	CSCD
语种	英语
资助项目	[National Program on Key Basic Research Project of China] ; [Program of International Science and Technology Cooperation of China] ; [National Natural Science Foundation of China] ; [Key Laboratories Development Program of Qinghai Province] ; [Qinghai-Utah Joint Research Key Lab for High Altitude Medicine] ; [Ministry of Education New Century Outstanding Talents Support Program of China] ; [Natural Science Foundation of Qinghai]
CSCD记录号	CSCD:5425432
引用统计	被引频次：5[CSCD] [CSCD记录]
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/143845
专题	中国科学院金属研究所
作者单位	1.中国科学院金属研究所 2.青海大学 3.Qinghai Entry Exit Inspect & Quarantine Bur, Xining 810001, Peoples R China
推荐引用方式 GB/T 7714	Gai Xiangyun,Wei Yuhai,Zhang Wei,et al. Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels[J]. ACTA PHARMACOLOGICA SINICA,2015,36(5):587-596.
APA	Gai Xiangyun.,Wei Yuhai.,Zhang Wei.,Wuren Tana.,Wang Yaping.,...&Ge Rili.(2015).Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels.ACTA PHARMACOLOGICA SINICA,36(5),587-596.
MLA	Gai Xiangyun,et al."Echinacoside induces rat pulmonary artery vasorelaxation by opening the NO-cGMP-PKG-BKCa channels and reducing intracellular Ca2+ levels".ACTA PHARMACOLOGICA SINICA 36.5(2015):587-596.