| Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats | |
| Alternative Title | Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats |
| Huang Q1; Shao L1; Jiang H1; Miao ZC1; Shi QD1; Liu SS1 | |
| 2001 | |
| Source Publication | ACTA PHARMACOLOGICA SINICA
 |
| ISSN | 1671-4083 |
| Volume | 22Issue:5Pages:455-458 |
| Abstract | AIM: To observe the effects of insulin on liver mitochondrial respiratory function, activity of H+-ATPase, and superoxide anion free radicals production in diabetic rats. METHODS: Rats were injected iv with alloxan 40 mg/kg to induce diabetes. The liver mitochondrial respiratory function was assayed by measurement of oxygen consumption using a Clark oxygen electrode. Superoxide anion production was assayed using chemiluminescence method. Activities of HC-ATPase were measured by luciferin-luciferase system and inorganic phosphorus's method. RESULTS: Insulin 1 U/kg sc daily for 9 weeks improved oxidative phosphorylation, respiratory rate state 3 (P 0.05). In the insulin group, synthesis activity of H+-ATPase was obviously increased (P < 0.05) acid hydrolytic activity of H+-ATPase was remarkably decreased (P < 0. 01), compared with the diabetes group. Insulin 1 U/kg for 9 weeks apparently decreased the production of O-2(.-)(P < 0. 01) in liver mitochondria of diabetic rats. CONCLUSION: Insulin can prevent the injury from superoxide anion in liver mitochondria, and improve the function of the liver mitochondria oxidative phosphorylation. |
| Keyword | insulin experimental diabetes mellitus mitochondria free radicals oxidative phosphorylation |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:515825 |
| Citation statistics |
Cited Times:1[CSCD]
[CSCD Record]
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| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/146311 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.中国科学院 2.中国科学院金属研究所 |
| Recommended Citation GB/T 7714 | Huang Q,Shao L,Jiang H,et al. Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats[J]. ACTA PHARMACOLOGICA SINICA,2001,22(5):455-458. |
| APA | Huang Q,Shao L,Jiang H,Miao ZC,Shi QD,&Liu SS.(2001).Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats.ACTA PHARMACOLOGICA SINICA,22(5),455-458. |
| MLA | Huang Q,et al."Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats".ACTA PHARMACOLOGICA SINICA 22.5(2001):455-458. |
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