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Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats
Alternative TitleEffect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats
Huang Q1; Shao L1; Jiang H1; Miao ZC1; Shi QD1; Liu SS1
2001
Source PublicationACTA PHARMACOLOGICA SINICA
ISSN1671-4083
Volume22Issue:5Pages:455-458
AbstractAIM: To observe the effects of insulin on liver mitochondrial respiratory function, activity of H+-ATPase, and superoxide anion free radicals production in diabetic rats. METHODS: Rats were injected iv with alloxan 40 mg/kg to induce diabetes. The liver mitochondrial respiratory function was assayed by measurement of oxygen consumption using a Clark oxygen electrode. Superoxide anion production was assayed using chemiluminescence method. Activities of HC-ATPase were measured by luciferin-luciferase system and inorganic phosphorus's method. RESULTS: Insulin 1 U/kg sc daily for 9 weeks improved oxidative phosphorylation, respiratory rate state 3 (P 0.05). In the insulin group, synthesis activity of H+-ATPase was obviously increased (P < 0.05) acid hydrolytic activity of H+-ATPase was remarkably decreased (P < 0. 01), compared with the diabetes group. Insulin 1 U/kg for 9 weeks apparently decreased the production of O-2(.-)(P < 0. 01) in liver mitochondria of diabetic rats. CONCLUSION: Insulin can prevent the injury from superoxide anion in liver mitochondria, and improve the function of the liver mitochondria oxidative phosphorylation.
Keywordinsulin experimental diabetes mellitus mitochondria free radicals oxidative phosphorylation
Indexed ByCSCD
Language英语
CSCD IDCSCD:515825
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/146311
Collection中国科学院金属研究所
Affiliation1.中国科学院
2.中国科学院金属研究所
Recommended Citation
GB/T 7714
Huang Q,Shao L,Jiang H,et al. Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats[J]. ACTA PHARMACOLOGICA SINICA,2001,22(5):455-458.
APA Huang Q,Shao L,Jiang H,Miao ZC,Shi QD,&Liu SS.(2001).Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats.ACTA PHARMACOLOGICA SINICA,22(5),455-458.
MLA Huang Q,et al."Effect of insulin on oxygen free radicals and oxidative phosphorylation in liver mitochondria of diabetic rats".ACTA PHARMACOLOGICA SINICA 22.5(2001):455-458.
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