| Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry | |
| Alternative Title | Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry |
| Dong QG1; Gu JK1; Zhong DF1; Fawcett JP1; Zhou H1 | |
| 2003 | |
| Source Publication | ACTA PHARMACOLOGICA SINICA
 |
| ISSN | 1671-4083 |
| Volume | 24Issue:6Pages:576-580 |
| Abstract | AIM: To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug. METHODS: SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method. RESULTS: Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs. CONCLUSION: The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47. |
| Other Abstract | AIM: To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug. METHODS: SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method. RESULTS: Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs. CONCLUSION: The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47. |
| Keyword | IN-VITRO HYDROXYLASE GLUCURONIDE LIVER SFZ-47 chicken embryo metabolism liquid chromatography electrospray ionization mass spectrometry |
| Indexed By | CSCD |
| Language | 英语 |
| CSCD ID | CSCD:1273872 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.imr.ac.cn/handle/321006/157904 |
| Collection | 中国科学院金属研究所 |
| Affiliation | 1.吉林大学 2.中国科学院金属研究所 3.University Otago |
| Recommended Citation GB/T 7714 | Dong QG,Gu JK,Zhong DF,et al. Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry[J]. ACTA PHARMACOLOGICA SINICA,2003,24(6):576-580. |
| APA | Dong QG,Gu JK,Zhong DF,Fawcett JP,&Zhou H.(2003).Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry.ACTA PHARMACOLOGICA SINICA,24(6),576-580. |
| MLA | Dong QG,et al."Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry".ACTA PHARMACOLOGICA SINICA 24.6(2003):576-580. |
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