IMR OpenIR
Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry
Alternative TitleMetabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry
Dong QG1; Gu JK1; Zhong DF1; Fawcett JP1; Zhou H1
2003
Source PublicationACTA PHARMACOLOGICA SINICA
ISSN1671-4083
Volume24Issue:6Pages:576-580
AbstractAIM: To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug. METHODS: SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method. RESULTS: Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs. CONCLUSION: The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47.
Other AbstractAIM: To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug. METHODS: SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method. RESULTS: Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs. CONCLUSION: The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47.
KeywordIN-VITRO HYDROXYLASE GLUCURONIDE LIVER SFZ-47 chicken embryo metabolism liquid chromatography electrospray ionization mass spectrometry
Indexed ByCSCD
Language英语
CSCD IDCSCD:1273872
Citation statistics
Document Type期刊论文
Identifierhttp://ir.imr.ac.cn/handle/321006/157904
Collection中国科学院金属研究所
Affiliation1.吉林大学
2.中国科学院金属研究所
3.University Otago
Recommended Citation
GB/T 7714
Dong QG,Gu JK,Zhong DF,et al. Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry[J]. ACTA PHARMACOLOGICA SINICA,2003,24(6):576-580.
APA Dong QG,Gu JK,Zhong DF,Fawcett JP,&Zhou H.(2003).Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry.ACTA PHARMACOLOGICA SINICA,24(6),576-580.
MLA Dong QG,et al."Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry".ACTA PHARMACOLOGICA SINICA 24.6(2003):576-580.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Dong QG]'s Articles
[Gu JK]'s Articles
[Zhong DF]'s Articles
Baidu academic
Similar articles in Baidu academic
[Dong QG]'s Articles
[Gu JK]'s Articles
[Zhong DF]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Dong QG]'s Articles
[Gu JK]'s Articles
[Zhong DF]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.