Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry

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	Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry
其他题名	Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry
	Dong QG 1; Gu JK 1; Zhong DF 1; Fawcett JP 1; Zhou H 1
	2003
发表期刊	ACTA PHARMACOLOGICA SINICA
ISSN	1671-4083
卷号	24 期号:6 页码:576-580
摘要	AIM: To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug. METHODS: SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method. RESULTS: Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs. CONCLUSION: The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47.
其他摘要	AIM: To develop an alternative method for investigation of drug metabolism by fertilized chicken eggs using 3H-1,2-dihydro-2-(4-methyl-phenylamino) methyl-1-pyrrolizinone (SFZ-47) as a probe drug. METHODS: SFZ-47 (15 mg) was injected into the albumen of eggs from standardized breed chickens previously incubated for 10 d. After 72 h of further incubation, the allantoic liquid was subjected to solid phase extraction on XAD-2 columns and analyzed by liquid chromatography-electrospray ion trap mass spectrometry method. RESULTS: Three major metabolites were identified, namely 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino) benzyl alcohol (SFZ-47-OH), 4-(3H-1,2-dihydro-1-pyrrolizinone-2-methyl-amino)-benzoic acid (SFZ-47-COOH), and its glucuronide conjugates. The metabolic profile was little different from that previously found in rabbits and dogs. CONCLUSION: The result demonstrates the usefulness of the fertilized chicken egg as a convenient source of both phase I and phase II metabolites for further metabolism studies of SFZ-47.
关键词	IN-VITRO HYDROXYLASE GLUCURONIDE LIVER SFZ-47 chicken embryo metabolism liquid chromatography electrospray ionization mass spectrometry
收录类别	CSCD
语种	英语
CSCD记录号	CSCD:1273872
引用统计
文献类型	期刊论文
条目标识符	http://ir.imr.ac.cn/handle/321006/157904
专题	中国科学院金属研究所
作者单位	1.吉林大学 2.中国科学院金属研究所 3.University Otago
推荐引用方式 GB/T 7714	Dong QG,Gu JK,Zhong DF,et al. Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry[J]. ACTA PHARMACOLOGICA SINICA,2003,24(6):576-580.
APA	Dong QG,Gu JK,Zhong DF,Fawcett JP,&Zhou H.(2003).Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry.ACTA PHARMACOLOGICA SINICA,24(6),576-580.
MLA	Dong QG,et al."Metabolism of SFZ-47 in chicken embryo by liquid chromatography-electrospray ion trap mass spectrometry".ACTA PHARMACOLOGICA SINICA 24.6(2003):576-580.